Characteritation of Streptococcus agalctiae from subclinical mastitis cases in dairy cow
Boky Jeanne Tuasikal, Tri Handayani, Dadang Priyoatmojo, Afi Candra Trinugraha, Totti Tjiptosumirat

Centre of Isotopes and Radiation Application/ CIRA, National Nuclear Anergy Agency

Abstract

Subclinical mastitis (SCM) is a disease that often infects dairy cow, which then reduce milk production. Streptococcus agalactiae is well known as a dominant causative agent of SCM. An experiment to obtain characteristic of S. agalctiae from subclinical mastitis cases in dairy cows has been conducted. These isolates were examined using presumptive S. agalactiae of Christie, Atkins, and Munch-Petersen (CAMP) test and followed by Grouping test to identified it, which was cultured in soft agar (SA) and serum soft agar (SSA) to observed the capability at surface phenotype expression. Fifty seven positive Gram and negative catalase coccal bacteries were isolated from the process of identification, of which 34 were CAMP positive. From the serogrouping test of these, 14 isolates of S. agalactiae Group B were identified. Furthermore, 8 isolates are diffuse cultured in SA, while the remain 6 isolates are compact in SSA media.

Keywords: Subclinical mastitis, S. agalactiae, CAMP and grouping test.

Topic: Material Science