Removal of Crude Oil by Thiobacillus sp. and Clostridium sp. at various temperatures and concentration of pollutant in liquid media
I.P. P. Primadani, A Rinanti *

Department of Environmental Engineering, Faculty of Landscape Architecture and Environmental Technology, Universitas Trisakti, Jakarta, Indonesia

Abstract

The activities of the petroleum mining industry in Indonesia have caused many problems of hazardous waste pollution and would otherwise conflict with plant, animal or human life. One technique for applying this return is using the Landfarming technique. The purpose of this study is to overcome the pollution of crude oil in the environment by using biotechnology through the process of bioremediation by enzymatic. This research was conducted in 6 stages. The parameters that discuss to determine the optimal environmental conditions that provide the highest efficiency for TPH degradation processes such as temperature with a variation of 25 – 45 oC, then the concentration of pollutants with a variation of 4% – 20%, and the contact time carried out in solid media with variations amounting to 3 – 21 days. After conducting research, research that produces the bacterium Thiobacillus sp. and Clostridium sp. under optimal conditions at 35oC with a pollutant concentration of 12% and a contact time of 14 days. Thus, it can be concluded that the bacterium Thiobacillus sp. and Clostridium sp. can set aside hydrocarbon compounds with a removal efficiency of 82%. So, this research needs to be done to study other physical parameters.

Keywords: bioremediation, landfarming, hydrocarbon, total petroleum hydrocarbon

Topic: Environmental Engineering