Gas Production Kinetics and Organic Matter Digestibility in Vitro of Diets Supplemented by Biochar and Liquid Smoke
Novia Qomariyah (1,2,*),Yuli Retnani (3), Anuraga Jayanegara (3), Elizabeth Wina (4), Idat G Permana (3)

1) Graduate School of Nutrition and Feed Science, Faculty of Animal Science, IPB University, Jl. Agatis Kampus IPB Dramaga Bogor 16680, Indonesia
2) South Sulawesi Assessment Institute for Agricultural Technologi (South Sulawesi AIAT), Makassar 90243, Indonesia
(*) novia_joyo[at]yahoo.com
3) Department of Nutrition and Feed Technology, Faculty of Animal Science, IPB University, Jl. Agatis Kampus IPB Dramaga Bogor 16680, Indonesia
4) Indonesian Research Institute for Animal Production, Ciawi Bogor 16720, Indonesia

Abstract

This research aimed to evaluate the use of biochar and liquid smoke as feed additives on gas production and organic matter digestibility in an in vitro rumen fermentation system. Treatments consisted of: control (substrate), biochar BC1 (0.1 mg / ml), BC2 (0.2 mg / ml), BC3 (0.3 mg / ml), BC4 (0.4 mg / ml), liquid smoke LS1 (0.25 ul / ml), LS2 (0.5 ul / ml), LS3 (0.75 ul / ml) and LS4 (1 ul / ml). The samples were further incubated in vitro with buffered-rumen fluid in four replicates, represented by two incubation units per replicate, and conducted for 48 h at 39oC. Parameters measured in the in vitro evaluation were total gas production and digestibility of organic matter. Data were tested using analysis of variance (ANOVA) and if there was a significant difference at P<0.05 then continued with Duncan’s multiple range test. The results showed that supplementation of biochar and liquid smoke did not affect gas production. However, the addition of biochar increased the digestibility of dry matter and organic matter as compared to control substrate (P <0.05). It was concluded that biochar supplementation and liquid smoke could be strategically used to increase feed digestibility.

Keywords: gas production, digestibility, diets, biochar, liquid smoke

Topic: Material Engineering