Elastase Inhibitory Activity of Methanol Extract and n-Hexane Extract of Garcinia xanthochymus Pericarp
Neneng Siti Silfi Ambarwati(a), Berna Elya(b)*, Yesi Desmiaty(c), Dwi Atmanto(a), Islamudin Ahmad(d)

a) Department of Cosmetology, Faculty of Engineering, Universitas Negeri Jakarta, Jl. Rawamangun Muka, Pulogadung, Jakarta Timur, DKI Jakarta, 13200, Indonesia
b) Department of Pharmacognosy-Phytochemistry, Faculty of Pharmacy, Universitas Indonesia, Depok, 16424 West Java, Indonesia
c) Department of Phytochemistry, Faculty of Pharmacy, Universitas Pancasila, Jakarta, 12640, Indonesia.
d) Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Mulawarman, Samarinda, East Kalimantan, Indonesia

Abstract

Garcinia xanthochymus is a plant found in Indonesia and rich in quercetin. Ethanol extract of bark can reduce LDL levels. Xanthon from the G. xanthochymus has antidiabetic activity. This study aims to determine the anti-elastase activity of n-hexane extract and methanol extract of G. xanthochymus pericarp. Samples were macerated for 1 time 24 hours using n-hexane and methanol respectively, while the elastase enzyme inhibition test used spectrophotometry methods by reacting extracts with the porcine pancreatic elastase enzyme and using the substrate N-Succ-(Ala)-3-p-nitroanilide (SANA). Absorbance was measured at wavelength of 405 nm with a microplate reader. The results showed that the concentration of 100 ppm extract of n-hexane extract of pericarp had enzyme inhibiting activity 65.17 ± 6.44%, methanol extract of pericarp 29.81 ± 10.67%, while the inhibitory activity of quercetin elastase enzyme as positive control was 62.75 + 1.89%. The conclusion of this research is that the n-hexane extract of the pericarp of G. xanthochymus has an activity as an inhibitor of the enzyme elastase is very high and can potentially be used as a material for making cosmetics.

Keywords: Garcinia xanthochymus, pericarp, anti-elastase,

Topic: Chemistry