The effect of Naphthalene Acetic Acid (NAA), Indole Butyric Acid (IBA) on the induction of Nepenthes plant (Nepenthes ventricosa) by in vitro
liberty chaidir,Hindun, Ari, Yati Setiati

Uin Sunan Gunung Djati Bandung

Abstract

Nepenthes ventricosa is a unique plant that has a bag on the tip of its leaves. This plant is included in CITES Appendix II or is experiencing scarcity. The scarcity is caused by giant exploration from society, so its efforts need to be made to increase this plant. One of the efforts for increasing this plant is by plant tissue isolation method. The purpose of this research was to determine the concentration of auxin types most effective against the growth of pitcher plant shoots (Nepenthes ventricosa) by in vitro. This research was conducted in January to June 2019 at Integrated Laboratory of UIN Sunan Gunung Djati Bandung. The material used was the Nepenthes ventricosa planlet was 14 months old on ¼ MS media.This research used RAL and descriptive method which consists of 7 treatments, i.e k, n1, n2, n3, i1, i2 and i3 which are repeated 4 times. The results of this research indicate that auxin has no real effect but NAA with a concentration of 0.1 ppm has a tendency to produce the fastest 11 HST shoots with a 2.3 mm shoot length and 1.3 shoots / explant shoots and the fastest 17 HST leaves, while NAA 0.2 ppm has a tendency towards the number of leaves 5.8 leaves / explants, 5.8 pitcher / explants, showing the fastest 14 HST roots with 4 mm root length and 2 root/ explants.

Keywords: Indole Butyric Acid, in vitro, Naphthalene Acetic Acid, Nepenthes ventricosa

Topic: Environmental Engineering