THE EFFECT OF PHOTOPERIODICITY VARIATIONS ON THE GROWTH OF Chlamydomonas WHICH IS GROWN IN BASAL BOLD MEDIUM
Nining Betawati Prihantini1*, Nelly Sari1, Wisnu Wardhana1, Dalia Sukmawati2

1Department of Biology, Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Indonesia, Kampus UI, Depok 16424, Indonesia
2Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Rawamangun, Jakarta Timur, Indonesia

Abstract

The research on the effect of various photoperiodicity on the cell density of Indonesia Indigenous Chlamydomonas has been done for 10 days observation. The research was experimental study of randomize design with eight treatments. The treatments were illumination period (photoperiodicity) of 0 L/ 24 D (0 Light/ 24 Dark), 8 L/ 16 D, 10 L/ 14 D, 12 L/ 12 D, 14 L/ 10 D, 16 L/ 8 D, 18 L/ 6 D, and 24 L/ 0 D. Photoperiodicity influence the growth of Subang isolated Chlamydomonas. Photoperiodicity of 24 L/ 0 D produced the highest cell density (6,729,250 cell/ml) on the day of eight. Photoperiodicity of 0 L/ 24 D produces the lowest cell density (191,100 cell/ml) on the day of 10. Kruskal-Wallis test showed there were effect of different photoperiodicity to cell densities of Chlamydomonas (p>0,05). Multiple Comparisons test to the average of cell density between the treatments showed there were absolutely differences (p>0,01) except between the treatment of 10 L/ 14 D (treatment 3) and of 12 L/ 12 D (treatment 4). Linear Regression test showed that photoperiodicity influences the logarithm of cell densities which was showed by y = 5,695 + 0,159x.

Keywords: Chlamydomonas; photoperiodicity; cell density

Topic: Environmental Engineering