Micro Capillary Columns Packed for Determination of Inorganic Ions in Biomaterials
Syukrya Ningsih*, Lee Wah Lim, Toyohide Takeuchi

1. Department of Chemistry, Suthan Thaha Saifuddin State Islamic University (UIN), Jambi, Indonesia
2. Department of Chemistry, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193

Abstract

Ion Chromatography is widely used analytical technique for determination of anionic and cationic analytes in various sample matrices. Culumns of flexible fused-silica tubing packed with commercially available materials have been prepared. The parameters which affect the chromatographic retention behavior have been examined. For investigation of the optimum concentration of the eluent, the NaCl and KCl concentration was varied from 50 to 25 mM and operated at eluent flow rate 3 µL/min. The microcolumns were prepared in the laboratory from fused silica tubing TSKgel IC-Anion-PW_XL (100 mm long x 0,320 I.D). The developed technique was successfully applied to the simultaneous determination of inorganic anions in real samples.

Keywords: micro packed column, ion chromatography, biomaterials

Topic: Chemistry