Effect of Growth Regulator 2,4-D and Kinetin on Durian (Durio zibethinus Murr.) Cimahi Landrace Bud Explant In Vitro
Agung Rahmadi (a), Noladhi Wicaksana (b), Bambang Nurhadi (c), Erni Suminar (b), Liberty Chaidir (d), Siti Rakhmah Tenrisui Pakki (e) dan Syariful Mubarok (a)*

(a) Master Agronomy Program, Faculty of Agriculture, Universitas Padjadjaran
(b) Departement of Agricultural Cultivation, Faculty of Agriculture, Universitas Padjadjaran
(c) Departement Food Technology, Faculty of Agricultural Industrial Technology, Universitas Padjadjaran
(d) Agrotechnology, Faculty Sains and Technology, Universitas Islam Negeri Sunan Gunung Djati
(e) Food and Agriculture Service, Cimahi City
(a) Correspondence: syariful.mubarok[at]unpad.ac.id

Abstract

Kamajaya Durian (Durio zibethinus Murr.) is one of rare tropical fruit plants placed in Cimahi. The parent tree of Kamajaya durian is approx. 120 years old. As a landrace plant, it needs an effort to maintain its quality and quantity. This study was conducted to determine the effect of 2,4-D growth regulators and Kinetin to form callus on Kamajaya durian bud leaves in vitro. The research was conducted at the Plant Tissue Culture Laboratory of the Faculty of Agriculture, Universitas Padjadjaran, from October to December 2019. The research used factorial randomized block design in three replication and descriptive analysis method. The first factor was growth regulator 2,4-D consisted of 5 treatments i.e. 1 ppm, 2 ppm, 3 ppm, 4 ppm, and 5 ppm. While the second factor was Kinetin concentrations i.e. 0 ppm, 0.5 ppm, and 1 ppm. The results showed 2,4-D of 2 ppm, 3 ppm, 4 ppm, and 5 ppm as well as combination between Kinetin 0.5 ppm + 2,4-D 2 ppm, Kinetin 0.5 ppm + 2,4-D 5 ppm and Kinetin 1 ppm + 2,4-D 5 ppm gave an effect of bud leaves explant curled.

Keywords: 2,4-D, Durian Kamajaya, Kinetin, Tissue Culture.

Topic: Material Science