COMPOSITION OF SOME FLAVONOID COMPOUNDS OF Selaginella SPECIES IN MOUNT MERAPI NATIONAL PARK AREA
Sri Rahayu, Isfi Zahara, Rahmah Aulia Azzahrah, Hafidzah Zahratunnisa, Farid Pujiono

Department of Biology, Faculty of Mathematics and Natural Science, Universitas Negeri Jakarta, Indonesia

Abstract

Selaginella is one of the genera of terrestrial plants that grow in humid area and can be used as a medicinal plant because it contains flavonoid such as biflavonoid. This substance are antioxidants and can prevent cancer. Mount Merapi National Park is an area protected by terrestrial ecosystems with an environment that supports the growth of fern plants such as Selaginella. This study aims to determine the levels of flavonoids of Selaginella species in the Kali Kuning and Kaliurang area of Mount Merapi National Park. Seven sample were obtained from field observation. The seven samples identified as S. opaca, S. plana, S. involvens, SP 1, and SP 2 in Kaliurang region and S. involvens and S.eurynota iin Kalikuning region. Highest water content were found in S. opaca (62%) and the lowest water content on S. plana (27%). Samples are extracted using acetone by participating 1: 6 for 48 hours. The highest yield found in S. eurynota (57.89%) while the lowest yield found in Selaginella SP 1 (12%). Qualitative detection of flavonoid content was done using TLC test. The eluent used consisted of ethanol:chloroform (1: 9) and showed visible yellow result. All Selaginella samples positive contain flavonoids. Measurement of flavonoid levels using ascorbic acid as a standard with the formula 2, 4, 6 and 8 ppm. The largest flavonoid content obtained in S. plana (69.283%) while the lowest level was obtained in S. eurynota (18.371%).

Keywords: flavonoid content, water content, extraction, TLC test

Topic: Chemistry