Bioremediation of petroleum hydrocarbon by hydrocarbono-clastic bacteria using landfarming techniques A Suwardi, R Ratnaningsih, A Rinanti*
Environmental Engineering Department, Faculty of Landscape Architecture and Environmental Engineering, Universitas Trisakti, Jakarta, Indonesia
Abstract
The intention of this research is to overcome soil pollution by petroleum using bioremediation method with the help of hydrocarbonoclastic bacteria. The first step in this research was to characterize petroleum as pollutants and cultivate mixed culture bacteria, i.e. Pseudomonas aeruginosa and Brevibacterium sp. The next step was degradation test with growth medium of SMSS (Stone Mineral Salt Solution) with variations in temperature and pH. It was conducted in a 300 ml erlenmeyer series with composition of 80% media, 5% petroleum, and 10% bacteria placed in an incubator shaker with a rotation speed of 200 rpm for 7 days. Based on the results from degradation test with growth medium of SMSS, obtained results that TPH (Total Petroleum Hydrocarbon) degradation efficiency is higher at a temperature of 40oC that is equal to 34.2%. In the variation of pH, the highest efficiency in TPH degradation occurs at pH 8 which is 39.4%. This research proves that Pseudomonas aeruginosa and Brevibacterium sp. can degrade hydrocarbon composition in petroleum with an efficiency of up to 30%.
Keywords: biodegradation, landfarming, hydrocarbon, petroleum, total petroleum hydrocarbon
