GENETIC DIVERSITY AND DIFFERENTIATION OF CYTOCHROME OXIDASE SUBUNIT I (COI) GENE OF Rasbora lateristriata Bleeker IN DIFFERENT HABITAT Sang Ayu Made Putri Suryani1, I Gede Putu Wirawan2, Rindang Dwiyani2, Made Sritamin2
1 Aquatic Resources Management department, Agriculture Faculty, Warmadewa university
2 Faculty of Agriculture, Udayana University, Denpasar, Indonesia
Abstract
Rasbora lateristriata is a freshwater fish have small size and schooling fish in the shallow river. This fish is called Nyalian by the people in Bali. The population of Rasbora lateristriata has decreased in upstream at Sungi river that already lightly polluted. The genetic diversity of Rasbora lateristriata based on the Cytochrome Oxidase subunit I gene as a marker will be detected in the upstream, branch river, middle stream and downstream of the Sungi river. The method that used is the polymerase chain reaction (PCR) with two primers are Fish-BCL (forward) and Fish-BCH (reverse) were followed by sequencing using the method of Sanger sequencing to obtain its nucleotide sequences. Genetic diversity was analyzed using Mega 5.2 version. Nucleotide composition of Rasbora lateristriata are Thymine 29.8%, Cytosine 25.8 to 26.2%, Adenine 26.0% and Guanine 18.5-18.6% with total nucleotide are 633 to 682. The branch of the river, middle stream and downstream which the genetic diversity of locus COI experienced a change of nucleotide at the sixth base, Thymine (T) is replaced with Cytosine (C). Substitution Thymine to Cytosine is occurring on a polluted part of the river that may be caused by the parameters of the water quality.
Keywords: Rasbora lateristriata, COI, Genetic diversity, water quality
