Acid Mine Drainage Removal by Mixed Bacteria Culture of Pseudomonas aeruginosa and Brevibacterium sp A N Putri, R Ratnaningsih and A Rinanti*
Departement of Enviromental Engineering, Faculty of Landscape Architecture and Enviromental Technology, Universitas Trisakti, Jakarta, Indonesia
Abstract
Acid mine water is a problem faced by the mining industry in Indonesia because it has a negative impact on the environment because it is very acidic and contains heavy metals. This bioremediation research to determine the ability of a mixed bacteria culture of Pseudomonas aeruginosa and Brevibacterium sp. in removal acid mine water. The study began with an allowance test in liquid media using artificial growth media Stone Mineral Salt solution (SMSs) with the tested parameters namely contact time (hours) and concentration of mine acid water pollutants (% v/v). Parameter variation consists of contact time (hours) 24 to 240 and concentration of acid mine pollutant water (% v/v) 10 to 25. Mixed culture are resistant to the presence of mine acid water as a substrate that is xenobiotic because no inhibition zones are formed around paper discs that have been exposed to acid mine pollutant water and can grow on Nutrient Agar (NA) media added with mine acid pollutants. The pH of the liquid media increased from 2.3 to 7.7 with a contact time of 144 hours with a temperature of 30 ℃ and a pollutant concentration of 10%. Based on the analysis with the Atomic Absorbsion Spectrophotometry (AAS) method, mixed culture of Pseudomonas aeruginosa and Brevibacterium sp. can eliminate iron and manganese metal content reaching 98% and 80%.
