Extraction and Characterization of Bixin from Anatto Seeds with microwave Erdawati and Edith Alanas
Universitas Negeri Jakarta
Abstract
This study aims to determine the effect of extraction time (5,10,15 minutes) and the ratio of the aquadest solvent: ethyl acetate (1: 6.5, 1: 7, 1: 8 v / v) to the yield of pigment . The optimum condition was found on the ratio of solvent of distilled water: ethyl acetate (1: 7 v / v) extraction time for 15 minutes with low microwave power resulted in maximum pigment yield of 0.469%. The isolate compounds were analyzed using IR spectroscopy, mass spectroscopy, and High Performance Liquid Chromatography (HPLC) showed that the isolates were bixin pigments having molecular weight of 394 g / mol with wave numbers for OH groups, C = O carboxylate groups, CH bonds, duplicate C = C, and CO ester bonds. As well as showing the retention time of standard and standard pigments at minutes to 12.849 and 12.843
