Simple Analytical Method Based on UV-Visible Spectroscopy Coupled with SIMCA for Authentication of Lampung Robusta Coffee with Geographic Indication (GIs) Meinilwita Yulia (a)*, Diding Suhandy (b), Sapto Kuncoro (b)
a)Department of Agricultural Technology, Lampung State Polytechnic, Jl. Soekarno Hatta No. 10, Rajabasa Bandar Lampung, 35141, Indonesia.
*meinilwitayulia[at]polinela.ac.id
b)Spectroscopy Research Group (SRG), Laboratory of Bioprocess and Postharvest Engineering, Department of Agricultural Engineering, Faculty of Agriculture, The University of Lampung, Jl. Prof. Dr. Soemantri Brojonegoro No.1, Bandar Lampung, 35145, Indonesia.
Abstract
Lampung Robusta coffee got a geographic indication (GIs) protection from Indonesian government since 2014 with certificate number of ID G000000026. This GIs coffee come from three different geographic origins: West Lampung, Tanggamus and Way Kanan. In this research, we evaluate the possible application of simple analytical method based on UV-visible spectroscopy coupled with SIMCA for authentication of Lampung Robusta coffee with GIs. Two types of Lampung Robusta coffee were used: GIs coffee from West Lampung and Tanggamus and non-GIs coffee from Pesawaran. Each type consists of 60 samples of ground roasted coffee with 1 g weight. The all samples were extracted using a hot distilled water and 3 mL aqueous samples were pipetted into 10 mm quartz cuvette. UV-visible spectral data were recorded in the range of 190-1100 nm. The PCA result showed that GIs coffee and non-GIs coffee can be well separated. The SIMCA classification was accepted with 100% of accuracy, sensitivity and specificity.