Antioxidant Activity of Potassium Aluminum Sulphate (Alum) as Medicine Preparation for Vaginal Discharge Titik Taufikurohmah(a*), Fadlilah Gita Shalli(a), Eka Apriyosa(b) and Suyatno(a)
(a) Departemen of Chemistry, Faculty of Mathematics and Natural Sciences, State University of Surabaya (UNESA), Indonesia
*Corresponding author : titiktaufikurohmah[at]unesa.ac.id
(2) PT. Khanza Ekselensia Utama, Indonesia
Abstract
Potassium aluminium sulphate (Alum), KAl(SO4)2.12H2O, is an inorganic salt with crystalline form. Alum has antioxidant activity that can play a role in treatment of excessive vaginal discharge. Excessive vaginal discharge can triggering an oxidative stress which marked by existance of reactive oxygen species (ROS), so that cause a cell death and tissue damage. This study aims to know the antioxidant activity of alum that can used as medicine preparation for vaginal discharge. The method used is the DPPH with concentration 0.003% and variation concentration of alum is 2; 1; 0.5; and 0.25 (%w/v). Then reduction at intervals of 30, 60, and 90 minutes and measured the absorbance using a UV-Vis Spectrophotometer at wavelength of 516nm. Inhibition percents and antioxidant activity determined through IC50 value. Result in this study showed high inhibition percent at high concentration. At time intervals of 30, 60, and 90 minutes reduction of DPPH showed the IC50 values that increased significantly of 5.2; 7; and 7.9. Based on the results can concluded that the higher concentration of alum the higher percent inhibition obtained and longer interaction between alum with free radicals cause decrease antioxidant activity proved by increasing IC50 value.
Keywords: Potassium Alum; Antioxidant; IC50; Vaginal Discharge; Medicine
