Variation in Recombinant Protein Production Volume of Fim-C Salmonella typhi as a Raw Material for Typhoid Detection Kit at Laboratory Scale M Nurjayadi1., S F Jinan1., T Setiyoto1., D Hardianto2., A Sulfianti2., K Agustini2., D Sukmawati3., HA El-Enshasy4
1 Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Negeri Jakarta Gedung KH. Hasjim Asj’ari, 6th Floor, Jl. Rawamangun Muka Jakarta Timur, 13220, Indonesia
2 Biofarmaka Research Laboratory LABTIAP BPPT-Serpong, Tangerang Selatan, Banten, Indonesia
3Department of Biology, Faculty of Mathematics and Natural Science, Universitas Negeri Jakarta Gedung KH. Hasjim Asj’ari, 9th Floor, Jl. Rawamangun Muka Jakarta Timur, 13220, Indonesia
4Institute of Bioproduct Development, Universiti Teknologi Malaysia (UTM), Skudai, Johor Bahru, Malaysia
Abstract
Salmonella typhi is gram-negative bacteria that caused typhoid fever in humans; the disease prevention is currently carried out by vaccination. In addition to vaccination, the development of disease detection tools is also being carried out so that the detection process is faster and more accurate. In line with the development of typhoid detection devices, previous studies have managed to find factors that influence the production of Fim-C S. typhi protein on a small scale as raw material for typhoid detection kits. The purpose of this research is to apply the results of previous studies in the production of recombinant Fim-C S. typhi proteins with volume variations of 50mL-300mL, as a foundation for large-scale production. The results of protein production were characterized by SDS-PAGE and protein concentration measurements using the BCA Assay at a wavelength of 562 nm. The results showed an amount of that protein increase along with gradually production volumes, mainly the protein in the form of inclusion bodies. Pursuant to the results obtained can be concluded production of the recombinant protein Fim-C-S. typhi at 50-300 mL volume variation on a laboratory scale has been successfully performed with consistent results, which is expected to be basic in production at pilot scale and large scale.
