Chemotaxonomic and Whole-Genome Characterization of an Actinobacterium Isolated from the Deep Sea
Chandra Risdian (a,b), Wiebke Landwehr (c), Manfred Rohde (d), Peter Schumann (e), Richard L. Hahnke (e), Cathrin Spröer (e), Boyke Bunk (e), Peter Kämpfer (f), Peter J. Schupp (g), and Joachim Wink (b)

a) Research Center for Environment and Clean Technology-National Research and Innovation Agency Republic of Indonesia (BRIN)

b) Microbial Strain Collection (MISG), Helmholtz Centre
for Infection Research (HZI), 38124 Braunschweig,
Germany
*Joachim.Wink[at]helmholtz-hzi.de

(c) Partner Site Hannover-Braunschweig, German Centre for
Infection Research (DZIF) , Braunschweig, Germany

(d) Central Facility for Microscopy, Helmholtz Centre for
Infection Research (HZI), 38124 Braunschweig, Germany

(e) Leibniz Institut DSMZ-German Collection of
Microorganisms and Cell Cultures, 38124 Braunschweig,
Germany

(f) Institut für Angewandte Mikrobiologie, Justus-Liebig-
Universität Giessen, 35392 Giessen, Germany

(g) Institute for Chemistry and Biology of the Marine
Environment, Carl von Ossietzky University of
Oldenburg, 26129 Oldenburg, Germany

Abstract

Nowadays, finding novel bioactive compounds, primarily from microbial sources, is very challenging. One of the strategies to solve this problem is to isolate the bacteria from the neglected and underexplored areas. The deep sea is one of the underexplored areas on Earth with the characteristics of extreme environments such as high pressure, low temperature, less oxygen concentration, and lack of light intensity. A novel actinobacterium, ASO4wetT, was isolated from the sponge in the deep sea collected in the North Atlantic Ocean. The 16S rRNA gene sequence analysis indicated that strain ASO4wetT was related to the genus Streptomyces and exhibited the closest similarities to Streptomyces karpasiensis K413T (98.87 %), Streptomyces glycovorans YIM M 10366T (98.38 %), and Streptomyces abyssalis YIM M 10400T (97.53 %). The primary menaquinone of strain ASO4wetT was MK-9(H8) and its major fatty acids were iso-C16:0, anteiso-C15:0, and iso-C15:0. The genomic DNA G + C content of strain ASO4wetT was 70.24 mol% with a genome size of 7,377,472 bp comprised of 6,332 coding sequences, 59 tRNA genes, and six rRNA operons.

Keywords: Chemotaxonomy, Deep-Sea Sponge, Streptomyces, The North Atlantic Ocean, Whole-Genome Analysis.

Topic: Biology