Isolation of dark septate endophyte (DSE) from ferns (Pteris vittata) roots
Dalia Sukmawati1,2*, Axel Mareta1, Atin Supiyani1, Shabrina Nida Al Husna3, Nuniek Ina Ratnaningtyas4, Hesham El Enshasy5,6 and Daniel Joe Dailin5

1 Biology Department, Laboratory of microbiology, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia- Dalia-Sukmawati[at]unj.ac.id
2.Universitas Negeri Jakarta Culture Collection, Laboratory of microbiology, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia- Dalia Sukmawati[at]unj.ac.id-
1 Biology Department, Laboratory of microbiology, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia, axelmareta[at]gmail.com
1Biology Department, Laboratory of microbiology, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia- umikhumaiya_1308617072[at]mhs.unj.ac.id
1Biology Department, Laboratory of microbiology, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia- atinsupiyani78[at]gmail.com
3 Department of Biology, Faculty of Biology, Jenderal Soedirman University, Dr. Suparno Road, Number 63, Karangwangkal, North Purwokerto, Banyumas Regency, Central Java, 53122, Indonesia. Email:fajarhusen001[at]gmail.com3
Department of Microbiology, School of Life Sciences and Technology, Institut Teknologi Bandung, Indonesia- shabrina.nida[at]gmail.com
4Institute of Bioproduct Development, Universiti Teknologi Malaysia (UTM), Skudai, Johor Bahru, Malaysia- henshasy[at]ibd.utm.my
4Institute of Bioproduct Development, Universiti Teknologi Malaysia (UTM), Skudai, Johor Bahru, Malaysia- , jddaniel[at]utm.my
5City of Scientific Research and Technology Applications, New Burg Al Arab, Alexandria, Egypt- henshasy[at]ibd.utm.my

Abstract

Dark septate endophytes (DSE) are a group of ascomycetes that live in intracellular and extracellular root tissue to facilitate plant growth and stress tolerance in extreme environments. However, little is known about the DSE fungi isolated from certain plant roots such as Pteris vittata, especially under drought condition. Pteris vittata is known for its ability to live in various types of substrates and ecosystems. In this study, we obtained DSE fungi from the fern roots Pteris vittata collected from the area of Universitas Negeri Jakarta. DSE isolation was carried out by inoculating the Pteris vittata fern roots with a size of 0.5 cm on the surface of sterilized PDA media for 5-7 days at 27&deg-C. Observations were made every 24h using a stereo microscope to see the first hyphae appeared from the plant roots. The results exhibited 13 samples of roots with hyphae colonization and were suspected to be DSE fungi. Of the 13 root isolates, only 3 isolate (PP2, PP4A and PPB) showed the DSE growth (23%) with melanin pigment. The morphological characteristics of endophytic DSE fungi collected from Pteris vittata roots represented septate hyphae, brownish to black colony color, and having a growing zone, and a velvety texture. For the isolate PP2, it showed sclerotia while for the isolate PP4, it exhibited light brown colonies.

Keywords: Pteris vittata, Dark septate endophyte (DSE), hyphae colonization

Topic: Biology