THE MATURASE K REGION OF KEBAK, A JAVANESE CULTURAL FLORA
Poppy Rahmatika Primandiri1,3, Muhammad Rifqi Hariri2, Tutut Indah Sulistiyowati1,3, Agus Muji Santoso1,3

1. Prodi Pendidikan Biologi, Universitas Nusantara PGRI Kediri
2. Pusat Riset Biosistematika dan Evolusi, Badan Riset & Inovasi Nasional
3. Kelompok Studi Biodiversitas, Universitas Nusantara PGRI Kediri

Abstract

Kebak is a historically significant plant in the Javanese cultural activity ^Ngunggahne Beras^. During Field Collection, several plants are morphologically indistinguishable, demanding the use of DNA sequences to back up the results of morphological identification. This research aims to describe the maturase k sequence and generate the first DNA barcode sequence of local kebak plants. The DNA was extracted from the leaves, amplified using matK universal primers, and sequenced. The morphological study identifies the kebak plant as Ficus fulva Reinw. ex Blume, a member of the Moraceae, the fig family. This species^ native range extends from northeast India to Malesia. This plant was distributed from Sumatra to Moluccas in Indonesia. It is a tree that performs well in the moist tropical biome. A length of 730 base pairs, partial region, of the matk Genes^ sequence was obtained. The sequence comparison through BLAST revealed that the sequence for Ficus fulva was unavailable, but it is 99.72% similar to Ficus esquiroliana H.Lév., a synonym for Ficus triloba Buch.-Ham. ex Voigt. Compared to four other Ficus species found in Java, the matK sequence revealed six diverse sites, suggesting that this region is predominantly conserved.

Keywords: conservation, cultural plant, kebak, matK

Topic: Biology