Analysis of nutrient and microbial content in ecoenzyme solution Hertien Koosbandiah Surtikanti(a*), Yanti Hamdiyati(b), Febby Nurfadilah(a)
a) Prodi Biologi FPMIPA UPI, jalan Dr. Setiabudi 229 Bandung
*hertien_surtikanti[at]yahoo.com
b) Prodi Pendidikan Biologi FPMIPA UPI, jalan Dr. Setiabudi 229 Bandung
Abstract
Ecoenzyme is a solution of organic substances resulting from the fermentation of molasses, variation of organic waste (vegetables and fruit peels), and water with the proportion of 1:3:10. The use of Ecoenzyme as an organic fertilizer has been studied, however study on nutrient and bacteria analysis is limited. Therefore, the aim of this study was to analyze the content of nutrient and microbial in ecoenzyme. Analysis of N-Total. P2O5 and K2O in ecoenzyme are done by using Titrimetri, Spectrophotometri and Photometri respectively. Meanwhile microbial analysis is done as following bacteria isolation, making pure cultures, observing colony morphology both macroscopically and microscopically and testing the biochemical activity of bacteria. Testing the biochemical activity including starch hydrolysis test, fat hydrolysis test, gelatin hydrolysis test casein test, catalase test, H2S production test, indole test, MR-VP test, test citrate, carbohydrate fermentation test, motility test, oxidase test, nitrate reduction test, and urease test. The data analysis stage is carried out after obtaining data on the characteristics of the bacteria, then identification of the bacteria was carried out based on Bergey^s Manual of Determinative Bacteriology Ninth Edition (1994). The results showed that ecoenzyme contained NPK nutrients, namely 0.38% N element, 0.0% P element and 0.15% K element. These levels are still very low to meet the standard requirements for liquid organic fertilizer. Six genera of bacteria in the ecoenzyme solution were identified based on the results of microscopic, macroscopic observations and biochemical tests. There are Pseudomonas, Brucella, Bacillus, Neisseria, Sulfidobacillus and Alcaligenes.
