Protective effect of Quercetin Administration in Bacterial-induced Periodontitis on Rats Afianti Sulastri (a,b*), Indra Mustika SP (c), Amaliya Amaliya (c), Sukrasno Sukrasno (d), Andreanus A. Soemardji (a)
a) Pharmacology-Clinical Pharmacy Research Group, School of Pharmacy, Institut Teknologi Bandung, Indonesia
b)Faculty of Sport and Health Education, Universitas Pendidikan Indonesia, 40154, Bandung, Indonesia
*afiantisulastri[at]upi.edu
c)Department of Periodontology, Faculty of Dentistry, Universitas Padjadjaran, 40132, Bandung, Indonesia
d)Pharmaceutical Biology-Research Group, School of Pharmacy, Institut Teknologi Bandung, Indonesia
Abstract
Previous studies had shown that quercetin pharmacological activity is beneficial for health. This study aims to investigate the effect of quercetin on bacterial-adhered ligature-induced periodontitis. The animals were divided into three experimental groups using two types of treatment, preventive (Qp, n=6), which is given a week before induction, and curative (Qc, n=6), which is given a week after induction. Periodontitis state was gained through placing a 3/0 non-resorbable sterile silk thread around the mandibular incisor teeth of eighteen male adult Sprague Dawley rats under general anaesthesia. Two weeks of post-induction treatment, the animals were euthanized, and samples representing the mandibular part were stored in formalin and prepared for histological processing to assess the grade of inflammation (GI). The clinical parameter was evaluated by measuring the periodontal pocket depth (PPD) using the Michigan-O probe. Qp showed the best improvement, in both parameter, clinically (PPD score, p=0,0018 at the lingual site, and p=0,0264 at the mesial site) and histologically (GI, p=0,0002). We found significant differences in preventing clinical attachment-loss statistically (p<0,05) on Qp, better than the Qc at an equal dose (p<0,05). This finding suggests that quercetin administered as a preventive measure may enhance the healing process of gingiva in periodontitis condition, better than other groups.
