THE EFFECT OF KECOMBRANG FLOWER (Etlingera elatior) EXTRACT ON MALE MICE (Mus musculus) SPERM QUALITY INDUCED BY CYCLOPHOSPHAMIDE Hernawati, Didik Priyandoko, Frizka Ayuningtyas
UNIVERSITAS PENDIDIKAN INDONESIA
Abstract
Kecombrang (Etlingera elatior) is one of the plants from Zingiberaceae family that widespread in Indonesia. Mostly people used this plant as a food and medicinal materials, this is because kecombrang contains antioxidants which is can protect body from free radicals. Cyclophosphamide is an immunosuppresive anti-cancer drug, but it has side effects in the form of toxicity in several organs, including the reproductive organs. This study purpose was to analyze the effect of kecombrang flowers extract on mices testicular weight and sperm quality which include sperm count and sperm abnormalities. Method that used in this study was a complete randomized series using 36 male mice which were divided into six groups (negative control, positive control, 5 mg/kg body weight, 10 mg/kg BW, 15 mg/kg BW and 20 mg/kg BW). Mices were acclimatized and then induced with cyclophosphamide 50 mg/kg body weight, then treated with kecombrang flower extract for 14 days using gavage needles. Statictical analysis using One-Way ANOVA test. The results showed that the most stable dose to keep sperm quality is 15 mg/kg body weight. This study shows that kecombrang flower extract can affect sperm quality, in this study is fix sperm quality and reduce the side effect of cyclophosphamid on reproductive system.
Keywords: Etlingera elatior, sperm quality, male mice, cyclophosphamide
