Use of Betalain Dyes Beetroot Extract (Beta vulgaris L.) as Alternative Dyes Safranin Substitute In Bacteria Simple Stain Anita Oktari (a*), Haidir Syafrullah (b), Nizar Khairumi (a)
a) Department of Medical Analyst, School of Analyst Bakti Asih, Bandung, Indonesia
*nio80zahra[at]gmail.com
b) Health Science School of Dharma Husada, Bandung, Indonesia
Abstract
Bit is a plant that has many benefits for health, is also beneficial if applied in the field of coloring mikrobiologi. The purpose of this research was to determine the root beet (Beta vulgaris L.) extract can be used as an alternative to the dye safranin on simple coloring in coloring Salmonella typhi and Staphylococcus epidermidis. Experimental research has been done by way of beet extraction using ethanol by maceration for 24 hours. Then the extract was concentrated using a rotary vacuum evavorator, then test for staining bacteria at a concentration of 5%, 7.5%, 10% and 12.5%. Observed using a microscope magnification 1000 times and the results were analyzed using statistical tests. Based on statistical test of Mann-Whitney to simple staining with a solution of beet extract at concentrations of 10% and 12.5% had a p-value> 0.005 which is 1.000 for both types of bacteria, meaning that no significant difference compared with 0.25% safranin as a control. Thus, the dye betalain beet extract concentrations of 10% and 12.5% can be used as an alternative to the dye safranin on simple coloring in coloring S. typhi and S. epidermidis because it produces the same color contrast control solution safranin 0.25 %. For further research is recommended to use other solvents and used the dried extract that can be longer stored.
