Modification of Pickle Goat Skin with Silver Nanoparticles by using Brown Algae (Padina sp.) with Assisted Microwave Eli Rohaeti1*, Amalia Sultan Nanda Annisa2, Rusita1, Kun Sri Budiasih1, and Nur Aeni Ariyanti1
Department of Chemistry Education FMIPA Universitas Negeri Yogyakarta Indonesia
Abstract
The objectives of this study were to determine effect of modification of goat skin with silver nanoparticles prepared by using brown algae (Padina sp.) and assisted microwave on hydrophobic properties, antimicrobial activity, mechanical properties, and biodegradability of goat skin. The synthesis of silver nanoparticles was carried out by using microwave method with bioreductor of brown algae extract (Padina sp.) and a stabilizer of a starch solution. Characterization of resulted silver nanoparticles was conducted by determining the wavelength utilizing the UV-Vis instrument and the particle size with Particle Size Analyzer (PSA). Characterization of the modified goat skin was conducted by measuring hydrophobicity through the sessile drop method, the antimicrobial activity test on modified goat skin was done by determining the clear zone against Escherichia coli (gram-negative bacteria) and Staphylococcus epidermidis (gram-positive bacteria), and also against the fungus (Candida albicans). The mechanical properties of modified goat skin were carried out by measuring the tensile strength of the skin. The statistical test was conducted to decide the modification effect on goat skin pickle. The characterization with UV-Vis on resulted silver nanoparticles revealed 426 nm wavelength absorption and diameter of silver nanoparticles by using PSA was 58.2 nm. As many as 99.25 % of silver nanoparticle has coated the surface of pickle goat skin. The modification of goat skin by adding silver nanoparticle can increase antibacterial activity, antifungi activity, and hydrophobicity of goat skin. The contact angle and the tensile strength of goat skin after modification was 81.49o and 14.63 MPa. The clear zone of goat skin after modification against Staphylococcus epidermidis, Escherichia coli, Candida albicans was 9.33 mm, 8.87 mm, and 8.79 mm, respectively. Meanwhile, the best biodegradability was the unmodified goat skin.
Keywords: Brown Algae, Silver nanoparticles, Goat Skin, Antimicrobial, Biodegradation