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1	Biology	ABS-5
Diversity of Pollinator Insects in the Vegetable Plantation in Kecamatan Dolat Rayat Febrina Suci Ramadhoni (a*), Syarifuddin (b), Mufti Sudibyo (b) a) Postgraduate Student of Biology Education Program, State University of Medan Jalan Willem Iskandar/Pasar V, Medan 2022, Indonesia *febrinasuci.fs[at]gmail.com b) Lecture of Biology Education Program, State University of Medan Abstract The presence of insect pollinators plays an important role in the pollination process. This is because the insects move the stamens from the anthers to the stigma, causing fertilization. The purpose of this study is to analyse the diversity of insect pollinators in vegetable plantations in 3 villages at Kecamatan Dolat Rayat, Kabupaten Karo. Sampling was carried out on five types of plantation: bean plant, chayote, peas, eggplant, and chilli. At each sampling point, five plots of 1,2m x 10m were established with a spacing of 2m between the plots. Insect pollinator observation technology using sampling scan method combined with insect net. The results of the study identified 7 families of 10 species and 880 individuals. Diversity index that used is The Shannon-Weiner with an average of H^= ± 1,20 in the villages. The most prevalent order was Apidae, and the most abundant species was Apis cerana. The greatest variety of pollen hybrids was found on chayote plantations. Flower anthesis is used as determinant of this observation. Keywords: Pollination- Fertilization- Shannon-Weiner index- Anthesis Share Link | Plain Format | Corresponding Author (Febrina suci Ramadhoni)

2	Biology	ABS-8
Organoleptic Analysis of Egytian Martabak With The Addition of Betung Bamboo Shoots (Dendrocalamus asper) Laili Suhairi (a*), Indani (a), Abdullah (b,c), Yuri Gagarin (c), Halimatussakdiah (a) a. Family Welfare Education Study Program (Tata Boga), Faculty of Teacher Training and Education, Syiah Kuala University. *lailisuhairi[at]unsyiah.ac.id b. Biology Education Study Program, Faculty of Teacher Training and Education, Syiah Kuala University. c. Research Center for Elephant Conservation and Biodiversity Forest Abstract Betung bamboo shoots (Dencrocalamus asper) is a plant derived from the shoots of bamboo betung processed into additional stuffing in Egyptian martabak. Betung bamboo shoots contain 59 g of phosphorus and 13 mg of calcium, so they are good sources of natural nutrients for the body. The purpose of the study was to determine the organoleptic characteristics of the Egyptian martabak from bamboo betung shoots and to determine consumer acceptance of the Egyptian martabak from bamboo betung shoots. This study used an experimental method with 3 treatments and 3 repetitions using a completely randomized design (CRD). Data collection techniques used observation tests conducted by 5 sources, acceptance tests were carried out by 30 consumers. The results of the observation test were analyzed by calculating the average value obtained from the sources, while the acceptance test was analyzed using one-way analysis of variance (ANAVA). The results of the observation by the interviewees chose the Egyptian martabak, bamboo shoots, treatment 3 with the code MM3 with the addition of 70 g of bamboo shoots, the average value was 23.31. The results of the research acceptance test consumers like the color, texture, taste of the Egyptian martabak bamboo shoots betung treatment 1 (MM1) with the addition of 50 g of bamboo shoots (25%), for the aroma consumers like Egyptian martabak bamboo betung shoots treatment 2 (MM2) with the addition of bamboo shoots bamboo betung 60 g (30%). The resource person chose the Egyptian martabak, bamboo shoots, treatment 3 with the code MM3 in terms of color, aroma, texture, taste. Consumers choose the Egyptian martabak bamboo shoots with treatment 1 (MM1) in terms of color, texture, and taste. Meanwhile, in terms of aroma, consumers chose the Egyptian martabak, bamboo shoots, treatment 2 (MM2). Keywords: Organoleptic Characteristics- Egyptian Martabak- Betung Bamboo Shoots Share Link | Plain Format | Corresponding Author (Laili Suhairi)

3	Biology	ABS-12
Selaginella of Merapi National Park and Their Antioxidant Capacity SRI RAHAYU, RAHMAH AULIA AZZAHRAH, HAFIDZAH ZAHRATUNNISA, ISFI ZAHARA, FARID PUJIONO Universitas Negeri Jakarta Abstract Many studies have been made to explore the potency of plants as candidates for antioxidants. The study investigates Selaginella found in Merapi National Park, Yogyakarta and their antioxidant Capacity. Exploration of the Kalikuning and Kaliurang area was done followed with qualitative and quantitative analysis. Six Selaginella samples were identified as Selaginella opaca, Selaginella plana, Selaginella involvens, SP 1, SP 2, and Selaginella eurynota. All samples positively contain flavonoid which are significantly different (Sig 0.001). Highest flavonoid was found in Selaginella plana (69,283 ± 0,04 mg QE/gr) and the lowest was in Selaginella eurynota (18,371 ± 7,48 mg QE/gr). Results clearly suggest that Selaginella can be used as a candidate for antioxidants. Keywords: selaginella, flavonoid, antioxidant, Merapi Share Link | Plain Format | Corresponding Author (Sri Rahayu)

4	Biology	ABS-16
Physical Characteristics of Bean and Biochemical Compounds for Komasti and Andungsari Arabica Coffee I Priantari1,3, Suratno1, D Wahyuni1 and Dafik2 1 Science Education Doctoral Program, Universitas Jember, Jl. Kalimantan No. 37, Jember 68121, Indonesia 2 Mathematics Education Masters Program, Universitas Jember, Jl. Kalimantan No. 37, Jember 68121, Indonesia 3Biology Education Program, Universitas Muhammadiyah Jember, Jl. Karimata No. 49, Jember 68121, Indonesia Abstract Coffee is one of the most consumed beverages in the world, and Indonesia is one of the largest coffee-producing countries. Arabica is the most popular among coffee lovers, specifically the variety of Andungsari and Komasti. Coffee lovers^ favorite product is determined by the Physical Quality Test and Biochemical Compound. Komasti and Andungsari varieties of arabica coffee used in this study were harvested in 2020. The Physical characteristic Quality Test used was based on the Indonesian National Standard (SNI) number 01-2907-2008, which consisted of the defect and rice coffee quality value, screen size coffee beans, coffee impurities, rancid beans and molds, insects, and water content (moisture). The roasting process also determines the levels of secondary metabolites and coffee aroma. The roasting process was carried out on Andungsari and Komasti with estimated roasting time of 2 minutes, 4 minutes, 6 minutes, 8 minutes, 10 minutes, 12 minutes, 14 minutes, 16 minutes, and 18 minutes. The results were, then, tested for chemical properties with caffeine content. Novelties from this study showed that the Arabica coffee of Komasti and Andungsari varieties showed physical quality tests at grades 1 and 2 and satisfy the quality specifications of SNI 01-2907-2008. The longer the roasting time for the Komasti and Andungsari varieties, the lower the caffeine content will be. All coffees obtained in this study were in the range of 0.45-2% caffeine and are all in accordance with SNI 01-3542-2004. Based on the EECA, Andungsari coffee roasted for 6-18 minutes and komasti 4-18 minutes is included in the EECA category Keywords: Komasti, Andungsari, Arabica Coffee Share Link | Plain Format | Corresponding Author (Ika Priantari)

5	Biology	ABS-33
Phytochemical Profile and Sensory Evaluation of Natural Vinegar from Mixed Fruits and Flowers of Melastoma Malabathricum L. with Variations in Starter Concentration and Fermentation Duration Safrida Safrida1, Gholib2, Maim Matualiah1, Fitrah Asma Ulhusna1* 1) Biology Education Department, Faculty of Teacher Training and Education, Syiah Kuala University, Darussalam-Banda Aceh 23111, (0651) 7551407 2) Laboratorium Fisiologi, Fakultas Kedokteran Hewan, Universitas Syiah Kuala, Banda Aceh, Indonesia Abstract Melastoma malabathricum L. is a wild plant in Aceh that can be used as traditional medicine. The aim of this research was to determine the phytochemical profile and sensory evaluation of the fruit and flower vinegar of Melastoma malabathricum L. This research used a completely randomized design (CRD) consisted of 2 main groups, saccharomyces cerevisae 5 days and 10 days with 12 treatments. Both groups were added with Acetobacter aceti concentrations of 5%, 10%, and 15% which were fermented for 14 days and 28 days. Phytochemical screening of vinegar includes the examination of flavonoids, saponins, tannins, quinones, polyphenols, steroids, and terpenoids. The results showed that the phytochemical test of the vinegar contained flavonoids, saponins, tannins, polyphenols, and terpenoids. The results of the best sensory evaluation test were P3 vinegar, namely the 5-day Saccharomyces fermented vinegar group, S1= starter (15% starter), 28 days of Acetobacter aceti fermentation with color, taste, aroma, and acceptance favored by the public. This natural vinegar product can be used as a drink that is beneficial to public health. Keywords: vinegar, Melastoma malabathricum L, organoleptic. Share Link | Plain Format | Corresponding Author (Fitrah Asma Ulhusna)

6	Biology	ABS-36
THE EFFECT OF YEAST ANTAGONIST ISOLATED FROM THE FERMENTATION OF COCOA BEANS (Theobroma cacao) FROM LAMPUNG, INDONESIA, ON THE GROWTH OF Aspergillus flavus UNJCC F-55 Dalia Sukmawati1,2, Alika Firhandini1, Siti Nurkhasanah1, Umi Khumaiya1, Atin Supiyani1, Shabrina Nida Al Husna3 ,Hesham El Enshasy4,5, Daniel Joe Dailin4, and Catur Sriherwanto6 1 Biology Department, Laboratory of microbiology, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia- Dalia-Sukmawati[at]unj.ac.id 2.Universitas Negeri Jakarta Culture Collection, Laboratory of microbiology, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia- Dalia-Sukmawati[at]unj.ac.id- 1 Biology Department, Laboratory of microbiology, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia- allika.firhandini.bioa17[at]gmail.com 1 Biology Department, Laboratory of Microbiology, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia- sitinurkhasanah68[at]gmail.com 1Biology Department, Laboratory of microbiology, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia- umikhumaiya_1308617072[at]mhs.unj.ac.id 1Biology Department, Laboratory of microbiology, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia- atinsupiyani78[at]gmail.com 3 Department of Microbiology, School of Life Sciences and Technology, Institut Teknologi Bandung, Bandung, Jawa Barat, Indonesia- shabrina.nida[at]gmail.com 4Institute of Bioproduct Development, Universiti Teknologi Malaysia (UTM), Skudai, Johor Bahru, Malaysia- henshasy[at]ibd.utm.my 4Institute of Bioproduct Development, Universiti Teknologi Malaysia (UTM), Skudai, Johor Bahru, Malaysia- Email: jddaniel[at]utm.my 5City of Scientific Research and Technology Applications, New Burg Al Arab, Alexandria, Egypt- Email:henshasy[at]ibd.utm.my 6Centre for Applied Microbiology, National Research and Innovation Agency (BRIN), Science and Technology Park, Banten, Indonesia- catur.sriherwanto[at]brin.go.id Abstract Mold is one of the microorganisms that causes damage to cocoa fruit because it produces mycotoxins as secondary metabolites that can lower the crop yield. One type of pathogenic mold that destroys cocoa fruit is Aspergillus flavus. A. flavus produces aflatoxin B1 which is the most potent hepatotoxin and carcinogen to humans. Amongst the most effective way to avoid alpha-toxin contamination in cocoa beans is to inhibit the growth of A. flavus in the beans. However, the use of pesticides and fungicides can increase the development of resistant strains of fungi and have a negative impact on the environment and human health. Therefore, we need other alternatives such as bio-control agents using antagonistic microorganisms, including yeasts. Thus, this study aimed at characterization of yeasts isolated from the fermented cocoa beans (Theobroma cacao) from Lampung, Indonesia, and evaluation of their ability in inhibiting the growth of Aspergillus flavus UNJCCF-55. The methods used were yeast screening, dual culture technique for antagonistic test, and morphological characterization. The 37º C-growth screening gave 89 out of 98 yeast isolates. The subsequent antagonist test resulted in 13 isolates with the highest inhibition zone against A. flavus UNJCCF-55. These yeast isolates were macroscopically of smooth surface, butyrous texture, milky white colour, irregular edges, and convex elevation. Microscopic observation showed the isolates having oval cell shape, asexual reproduction of budding, and non-hyphae structure Keywords: cocoa bean fermentation, yeasts, A. flavus, antagonistic activity Share Link | Plain Format | Corresponding Author (Dalia Sukmawati)

7	Biology	ABS-37
Antagonistic activity of cellulase-producing yeasts isolated from cocoa fermentation against pathogenic molds collected from damaged cocoa fruits Dalia Sukmawati1,2*, Alika Firhandini1, Umi Khumaiya1, D.A. Komsiatun1, Atin Supiyani1, Dwi Ningsih Susilowati3, Shabrina Nida Al Husna4, Hesham El Enshasy5,6, Daniel Joe Dailin5, and Catur Sriherwanto7 1Biology Department, Laboratory of microbiology, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia- Dalia-Sukmawati[at]unj.ac.id 2Universitas Negeri Jakarta Culture Collection, Laboratory of microbiology, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia- Dalia-Sukmawati[at]unj.ac.id- 1 Biology Department, Laboratory of microbiology, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia- allika.firhandini.bioa17[at]gmail.com 1Biology Department, Laboratory of microbiology, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia- umikhumaiya_1308617072[at]mhs.unj.ac.id 1Biology Department, Laboratory of microbiology, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia- atinsupiyani78[at]gmail.com 3Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Indonesia Jl. Tentara Pelajar No. 3A, Bogor 16111, Jawa Barat, Indonesia Telp. (0251) 8337975, 8354985- Faks. (0251) 8338820. Email: d_nengsusi[at]yahoo.com 4Department of Microbiology, School of Life Sciences and Technology, Institut Teknologi Bandung, Bandung, Jawa Barat, Indonesia- shabrina.nida[at]gmail.com 5Institute of Bioproduct Development, Universiti Teknologi Malaysia (UTM), Skudai, Johor Bahru, Malaysia- henshasy[at]ibd.utm.my 5Institute of Bioproduct Development, Universiti Teknologi Malaysia (UTM), Skudai, Johor Bahru, Malaysia- Email: jddaniel[at]utm.my 6City of Scientific Research and Technology Applications, New Burg Al Arab, Alexandria, Egypt- Email:henshasy[at]ibd.utm.my 7Centre for Applied Microbiology, National Research and Innovation Agency (BRIN), Science and Technology Park, Banten, Indonesia- Email: catur.sriherwanto[at]brin.go.id Abstract The presence of pathogenic molds in post-harvested cocoa beans poses a significant problem to most Indonesian farmers. Pathogenic molds secrete phytopathogenic toxins which cause rotting, seriously damaging the cocoa fruits. The common pathogenic molds found in damaged cocoa beans are Phytophthora palmivora and Phytophthora megakarya. These molds are so far controlled by using chemical pesticides, whose the long-term use is potentially harmful to human health and environment. Thus, healthier and safer alternative is needed, especially using biological agents such as yeasts. Some yeasts produce cellulose-degrading enzymes that can possibly break down the fungal cell wall which is composed mostly of the polysaccharides chitin and glucan. In this study, yeasts were isolated during the cocoa fermentation process, followed by screening for cellulolytic activity using direct planting and paste method, and subsequent antagonistic testing by double culture technique. Out of 128 yeast isolates, 77 possessed cellulase activity, with 6 of them having the highest activity index. Antagonistic activity test of these 6 isolates (C1.0.4, C1.1.3, C2.3.10, C2.3.14, C3.5.11, and C3.3.1) against the pathogenic molds resulted in isolate C3.3.1 showing the highest inhibition percentage from the 2nd to 8th day of incubation. Keywords: Antagonistic activity, yeasts, cocoa fermentation, Phytophthora palmivora Share Link | Plain Format | Corresponding Author (Dalia Sukmawati)

8	Biology	ABS-47
Isolation of dark septate endophyte (DSE) from ferns (Pteris vittata) roots Dalia Sukmawati1,2*, Axel Mareta1, Atin Supiyani1, Shabrina Nida Al Husna3, Nuniek Ina Ratnaningtyas4, Hesham El Enshasy5,6 and Daniel Joe Dailin5 1 Biology Department, Laboratory of microbiology, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia- Dalia-Sukmawati[at]unj.ac.id 2.Universitas Negeri Jakarta Culture Collection, Laboratory of microbiology, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia- Dalia Sukmawati[at]unj.ac.id- 1 Biology Department, Laboratory of microbiology, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia, axelmareta[at]gmail.com 1Biology Department, Laboratory of microbiology, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia- umikhumaiya_1308617072[at]mhs.unj.ac.id 1Biology Department, Laboratory of microbiology, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia- atinsupiyani78[at]gmail.com 3 Department of Biology, Faculty of Biology, Jenderal Soedirman University, Dr. Suparno Road, Number 63, Karangwangkal, North Purwokerto, Banyumas Regency, Central Java, 53122, Indonesia. Email:fajarhusen001[at]gmail.com3 Department of Microbiology, School of Life Sciences and Technology, Institut Teknologi Bandung, Indonesia- shabrina.nida[at]gmail.com 4Institute of Bioproduct Development, Universiti Teknologi Malaysia (UTM), Skudai, Johor Bahru, Malaysia- henshasy[at]ibd.utm.my 4Institute of Bioproduct Development, Universiti Teknologi Malaysia (UTM), Skudai, Johor Bahru, Malaysia- , jddaniel[at]utm.my 5City of Scientific Research and Technology Applications, New Burg Al Arab, Alexandria, Egypt- henshasy[at]ibd.utm.my Abstract Dark septate endophytes (DSE) are a group of ascomycetes that live in intracellular and extracellular root tissue to facilitate plant growth and stress tolerance in extreme environments. However, little is known about the DSE fungi isolated from certain plant roots such as Pteris vittata, especially under drought condition. Pteris vittata is known for its ability to live in various types of substrates and ecosystems. In this study, we obtained DSE fungi from the fern roots Pteris vittata collected from the area of Universitas Negeri Jakarta. DSE isolation was carried out by inoculating the Pteris vittata fern roots with a size of 0.5 cm on the surface of sterilized PDA media for 5-7 days at 27&deg-C. Observations were made every 24h using a stereo microscope to see the first hyphae appeared from the plant roots. The results exhibited 13 samples of roots with hyphae colonization and were suspected to be DSE fungi. Of the 13 root isolates, only 3 isolate (PP2, PP4A and PPB) showed the DSE growth (23%) with melanin pigment. The morphological characteristics of endophytic DSE fungi collected from Pteris vittata roots represented septate hyphae, brownish to black colony color, and having a growing zone, and a velvety texture. For the isolate PP2, it showed sclerotia while for the isolate PP4, it exhibited light brown colonies. Keywords: Pteris vittata, Dark septate endophyte (DSE), hyphae colonization Share Link | Plain Format | Corresponding Author (Dalia Sukmawati)

9	Biology	ABS-52
Potential of Coral Resilience Post Bleaching in 2016 in the Water of Ujung Seuke East Coast of Weh Island, Indonesia Aldyza, N1, Barus, T. A2, a), Mulya, M. B3, Sarong, M. A4 1, 2, 3 Department of Biology, Faculty of Mathematics and Natural Science, Universitas Sumatera Utara, Medan, North Sumatra, Indonesia 4 Faculty of Teacher Training and Education, Universitas Syiah Kuala, Banda Aceh, Indonesia a) Corresponding author: ternala[at]usu.ac.id Abstract Mass bleaching had ever occurred in 2010 in Southeast Asia waters such as Thailand, Malaysia, and Indonesia. One of the Indonesian waters experienced bleaching was Weh Island area, which then underwent bleaching again in 2016 and had impacts on the coral ecosystem. Substrate changes and coral cover conditions are noteworthy to determine the potential for coral recovery after bleaching. Therefore, the aim of the study is to measure the condition of coral cover and the potential for coral resilience after bleaching in 2016. Benthic data collection used the Point Intercept Transect method, while measuring coral resilience potential used the resilience index through coral cover indicators, fleshy seaweed, and rubble. The observation showed that the coral ecosystem in the waters of Ujung Seuke is in moderate condition, i.e. 34%. Coral resilience rates have a high recovery potential based on observed indicators, i.e. coral cover by 34%, fleshy seaweed by 2.83%, and rubble by 21.17%. Keywords: Coral reef, Bleaching, Resilience, Turf algae Share Link | Plain Format | Corresponding Author (Nadia Aldyza)

10	Biology	ABS-321
Structure and Dominance of Macrozoobenthos as Biomonitoring Instrument in the Reuleng River Leupung Aceh Besar Indonesia Afkar (a), M. Ali Sarong (b), Ternala A. Barus (c), Arlen Hanel Jhon (d), Miswar Budi Mulya (e*), Nadia Aldyza (f), Zulfikar (g) a) Faculty of Teacher Training and Education, Universitas Almuslim, Bireuen, Indonesia. a), c), d), e), f) Faculty of Mathemathics and Natural Sciences, Universitas Sumatera Utara, Medan, Indonesia. *Corresponding author: miswarbm_bio[at]usu.ac.id b), g) Faculty of Teacher Training and Education, Universitas Syiah Kuala, Banda Aceh, Indonesia. Abstract The Reuleng River is a mangrove area located in Aceh Besar district. Environmental changes that occur in the structure and composition of the mangrove ecosystem in the Reuleng River affect the macrozoobenthos conditions in the area. This study aims to look at the structure of the Makrozoobenthos community as a Biomonitoring instrument in the mangrove ecosystem area. The study was conducted from February 2021 to May 2021. Determination of the sampling location was carried out using the purposive sampling technique and sampling was determined in 3 areas, namely upstream, stream, and downstream. The results showed that there were 19 species grouped into 4 genera and 12 families. The Macrozoobenthos Diversity Index in the Reuleng River area ranges from 2.62. The species that dominate the mangrove ecosystem area is the Faunus ater species with a dominant index value (0.16) and the lowest dominant species in the mangrove ecosystem is Thalassina anomala (0.01). No specific species were found that dominate the area. Based on descriptive observations, there is a group of Makrozoobenthos that has a relationship with the psychochemical quality of the air. The results show that the balance of the community in the area is still vulnerable and experiencing the impact of environmental pressures. Keywords: Macrozoobenthos- Mangrove- Psychochemical- the Reuleng River Share Link | Plain Format | Corresponding Author (Afkar Afkar)

11	Biology	ABS-68
Conservation of Stingless Bees (Hymenoptera: Meliponini) in West Kalimantan, Indonesia Manap Trianto, Hari Purwanto, and R.C. Hidayat Soesilohadi Faculty of Biology, Universitas Gadjah Mada. Jl. Teknika Selatan, Sekip Utara, Bulaksumur, Sleman 55281, Yogyakarta, Indonesia Abstract Pollination is a critical for food production and human livelihoods in West Kalimantan. Currently, stingless bees are major pollination service provider to many agricultural crops. However, there is clear evidence of recent declines in stingless bees and it is urgent need to look for alternative and native pollinators. West Kalimantan is rich with native pollinator such as stingless bees. Currently, at least six species were inventoried and could be used to pollinate several agricultural crops such as starfruit, guava, citrus, mango, watermelon, durian, and coconut. Nowadays, global attention focuses on the threat of decreasing numbers of plants and pollination species, which have a direct impact on ecosystem function, especially to local and global stingless bee biodiversity. Due to the current evidence of the decreasing number of stingless bees accumulated in taxonomic and geographical scales, we decided to observe the role of stingless bees cultivation based on species and habitat approaches. Species biodiversity, population size, and growth rates are influenced by Spatio-temporal variations all of them have an important role in their vulnerability to extinction. Several issues to be considered before stingless bees can be utilized as pollination service provider in agricultural ecosystem. These issues included conservation of their species, capacity building and research on their biology, ecology and behavior. Out of these, six species - Tetragonula laeviceps, T. drescheri, T. biroi, Homotrigona apicalis, Heterotrigona itama, and Lepidotrigona terminata, were able to be utilized to pollinate several agricultural crops in West Kalimantan. Keywords: Conservation, Stingless Bees, West Kalimantan Share Link | Plain Format | Corresponding Author (Manap Trianto)

12	Biology	ABS-334
Monitoring the Diversity of Lizards and Snakes (Reptilia: Squamata), Along the Boyong-Code River Area, Province of Daerah Istimewa Yogyakarta Donan Satria Yudha (a*), Rury Eprilurahman (a), Hendy Eka Putra (b), Rachmat Aditama Dwija Putra (b), Sheilana Nugraha Muslim (b) (a) Laboratory of Animal Systematics, Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan Sekip Utara, Yogyakarta 55281, Indonesia. *donan_satria[at]ugm.ac.id (b) Herpetological Study Club, Faculty of Biology Universitas Gadjah Mada, Jl. Teknika Selatan Sekip Utara, Yogyakarta 55281, Indonesia. Abstract Reptile is a group of animal consists of testudines, crocodiles, lizards and snakes. Most reptiles are capable to occupy wide range of habitats with different environmental conditions. One of the preferred habitats for reptiles is river. Boyong-Code River is one of the rivers which flow across the Province of Daerah Istimewa Yogyakarta (DIY). Research on the diversity of lizards and snakes in the Boyong-Code River has been done in 2012. This research is aimed to acquire new data on the diversity of herpetofauna in the Boyong-Code River and compare it with 2012 data research. The comparison purpose is to monitor the species diversity dynamics after 5 years. The data of herpetofauna diversity were acquired using a combination of several methods, i.e., VES (Visual Encounter Survey), riverbank cruising and transect. Sampling area in 2012 and 2017 were divided three four locations, i.e., upstream, midstream, and downstream. Species diversity of lizards and snakes in the Boyong-Code River in 2017 is consisted of 8 species of lizards (lacertilians: squamates: reptiles) and 4 species of snakes (serpents: squamates: reptiles). Species diversity dynamics is equal for lizards in 2012 and 2017- meanwhile there were more species of snakes in 2012 compare to the 2017. Keywords: Monitoring- Diversity- Lizards- Snakes- the Boyong-Code River Share Link | Plain Format | Corresponding Author (Donan Satria Yudha)

13	Biology	ABS-357
Chemotaxonomic and Whole-Genome Characterization of an Actinobacterium Isolated from the Deep Sea Chandra Risdian (a,b), Wiebke Landwehr (c), Manfred Rohde (d), Peter Schumann (e), Richard L. Hahnke (e), Cathrin Spröer (e), Boyke Bunk (e), Peter Kämpfer (f), Peter J. Schupp (g), and Joachim Wink (b) a) Research Center for Environment and Clean Technology-National Research and Innovation Agency Republic of Indonesia (BRIN) b) Microbial Strain Collection (MISG), Helmholtz Centre for Infection Research (HZI), 38124 Braunschweig, Germany *Joachim.Wink[at]helmholtz-hzi.de (c) Partner Site Hannover-Braunschweig, German Centre for Infection Research (DZIF) , Braunschweig, Germany (d) Central Facility for Microscopy, Helmholtz Centre for Infection Research (HZI), 38124 Braunschweig, Germany (e) Leibniz Institut DSMZ-German Collection of Microorganisms and Cell Cultures, 38124 Braunschweig, Germany (f) Institut für Angewandte Mikrobiologie, Justus-Liebig- Universität Giessen, 35392 Giessen, Germany (g) Institute for Chemistry and Biology of the Marine Environment, Carl von Ossietzky University of Oldenburg, 26129 Oldenburg, Germany Abstract Nowadays, finding novel bioactive compounds, primarily from microbial sources, is very challenging. One of the strategies to solve this problem is to isolate the bacteria from the neglected and underexplored areas. The deep sea is one of the underexplored areas on Earth with the characteristics of extreme environments such as high pressure, low temperature, less oxygen concentration, and lack of light intensity. A novel actinobacterium, ASO4wetT, was isolated from the sponge in the deep sea collected in the North Atlantic Ocean. The 16S rRNA gene sequence analysis indicated that strain ASO4wetT was related to the genus Streptomyces and exhibited the closest similarities to Streptomyces karpasiensis K413T (98.87 %), Streptomyces glycovorans YIM M 10366T (98.38 %), and Streptomyces abyssalis YIM M 10400T (97.53 %). The primary menaquinone of strain ASO4wetT was MK-9(H8) and its major fatty acids were iso-C16:0, anteiso-C15:0, and iso-C15:0. The genomic DNA G + C content of strain ASO4wetT was 70.24 mol% with a genome size of 7,377,472 bp comprised of 6,332 coding sequences, 59 tRNA genes, and six rRNA operons. Keywords: Chemotaxonomy, Deep-Sea Sponge, Streptomyces, The North Atlantic Ocean, Whole-Genome Analysis. Share Link | Plain Format | Corresponding Author (Chandra Risdian)

14	Biology	ABS-380
The role of Indonesian Indigenous Cyanobacteria Culture Collection as an Ex-situ Conservation Effort and Microalgae Biodiversity Study Material Nining Betawati Prihantini (a*) a) Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Campus UI, Depok, 16424, Indonesia *nining[at]sci.ui.ac.id Abstract An exploratory research has been carried out on Indonesian indigenous Cyanobacteria in an effort to collect these isolates to be used as learning materials for microalgae biodiversity. Cyanobacteria are known as photosynthetic bacteria and prokaryotic microalgae. The members of the cyanobacteria found were isolated, cultured, identified, and collected. The location for sampling cyanobacteria is the UI Depok campus area which consists of water from small lakes (situ) and soil. The results showed that 22 strains of Cyanobacteria were isolated and cultured properly, and consisted of 12 genera/species, namely Merismopedia sp. (1 isolate), Synechococcus sp. (1 isolate), Limnothrix redekei (1 isolate), Oscillatoria sp.4 (1 isolate), Oscillatoria sp.5 (1 isolate), Pseudanabaena sp. (3 isolates), Nostoc sp. (4 isolates), Anabaena sp. (1 isolate), Scytonema sp. (1 isolate), Westiellopsis sp. (1 isolate), Stigonema sp. (1 isolate), and Hapalosiphon sp. (6 isolates). Twenty-two (22) strains were then collected and treated by subculture method. Of the 11 genera found, there are 3 genera that have been known as genera that have potential as sources of biobased materials, biofuels, biofertilizers, and others, namely Synechococcus, Nostoc, and Anabaena. Keywords: Cyanobacteria, prokaryotic microalgae, culture, collection, ex-situ conservation, subculture Share Link | Plain Format | Corresponding Author (Nining Betawati Prihantini)

15	Biology	ABS-381
Biomass Production of Subang Isolated Scenedesmus in Tube-shaped Photobioreactor with the Exposure of Audible Sound (Music): Photobioreactor Improvement Strategy Rubiantin Mesha Nauli Tambunan (a), Nining Betawati Prihantini (a*) a) Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, 16424, Depok, West Java, Indonesia *nining[at]sci.ui.ac.id Abstract The cultivation of Subang Isolated Scenedesmus in tube-shaped photobioreactors (PBRs) with the exposure of audible sound (music) was done. The study was done to know the effect of different music to the growth and culture appearance of Subang Isolated Scenedesmus. Subang Isolated Scenedesmus itself is Indonesia^s indigenous microalgae from Subang, West Java. This study was using three groups of Subang Isolated Scenedesmus PBRs. One group was control group and not exposed to any sound (Control-PBR), one group was exposed to music called ^Blues for Elle^ (PBR-A), and another group was exposed to music called ^Bloom^ (PBR-B). Based on 8 days of observation, it is known that Subang isolated Scenedesmus in PBR-A has the highest average cell density at the peak phase (1.81±0.16 x 105 cells/mL), highest growth rate (26.22 % per day), and most intense green color compared to Subang isolated Scenedesmus in PBR-B and Control-PBR. Related-Samples Friedman^s Two-Way ANOVA by Ranks test has proven that the average cell density value of Subang isolated Scenedesmus in Control-PBR, PBR-A, and PBR-B are not the same (α = 95%, sig = 0.013). Therefore, different audible sound (music) with certain intensity and frequency may affect the growth of Subang Isolated Scenedesmus differently. Keywords: Audible sound, Cell density, Growth, Music, Photobioreactor (PBR), Scenedesmus. Share Link | Plain Format | Corresponding Author (Rubiantin Mesha Nauli Tambunan)

16	Biology	ABS-382
Possible Usage of Various Microalgae Species from Rawa Besar Small Lake as Aquaculture Feedant Fadhlurrahman Maulana (a), Nining Betawati Prihantini (a*) a) Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, 16424 UI Depok, West Java, Indonesia *nining[at]sci.ui.ac.id Abstract One of Rawa Besar Small Lake^s roles for the surrounding community is as a fishing spot. Locals would often go fishing at the edges of the small lake. The ability of a lake to provide fishes to catch is partly due to the availability of food in the ecosystem, most importantly the microalgae community as the main producer in the freshwater food web. This concept is used in aquaculture as a way to obtain a more sustainable ingredient for feedant. Microalgae are used for aquaculture feedant due to its nutritional value. However, it is important to assess the safety and suitability of microalgae used for feedant due to the difference in toxicity and nutritional value of various microalgae species. It is also important to create a plan for a large-scale culture system suitable for various potential microalgae species, in order to produce algal meal feedstock in a viable and sustainable way. Observation of the water samples taken from Rawa Besar Small Lake shown that potential microalgae species like Kirchneriella and Arthrospira lives in Rawa Besar Small Lake. However, toxic microalgae species like Planktothrix has been found in said water sample as well. Isolation of potential species is necessary in order to produce a healthy feedant for aquaculture out of Rawa Besar Small Lake^s microalgae community. Keywords: Aquaculture, Biomass, Culture system, Feedant, Microalgae, Nutritional value, Rawa Besar Small Lake, Toxicity Share Link | Plain Format | Corresponding Author (Fadhlurrahman Maulana)

17	Biology	ABS-140
Analysis of Flies Density at The Integrated Waste Management Site Piyungan, Bantul Regency, Special Region of Yogyakarta Dila Hening Windyaraini (a*), Ratriana Kartikasari (a), Nayla Humaira Maheswari (a), Raden Roro Upiek Ngesti Wibawaning Astuti (a), Raden Wisnu Nurcahyo (b), Sitti Rahmah Umniyati (c), Suwarno Hadisusanto (a) a) Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Yogyakarta 55281, Indonesia * dila.windyaraini[at]ugm.ac.id b) Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jl. Fauna No.2 Karangmalang, Yogyakarta 55281, Indonesia. c) STIKES Wira Husada Yogyakarta, Jl. Babarsari, Caturtunggal, Depok, Sleman, 55281, Indonesia Abstract Flies are Arthropods that belong to the Diptera Order with their characteristic as segmented body. Flies are vectors of food borne disease that cause diarrhea, typhus, and myiasis. The existence of garbage is suitable for flies to live and continue their breeding. They can survive at the Integrated Waste Management Site (IWMS). This research aims were to find the density and identificate the species of flies at IWMS Piyungan and residental area around it. This research was conducted from July until September 2021. The flies were collected using sweep net and the density was calculated with fly grill. After collecting the flies, they were identified using Borror and Delongs Introduction To The Study of Insect identification book (Tripplehorn and Johnson, 2005). The diversity analysis was calculated by using Shannon-Wiener Diversity Index and the distribution of flies by using Morisita Index. This research showed that the density of flies at IWMS Piyungan and residental area were high with the value were 68 and 43.6, respectively. The diversity index at IWMS Piyungan was low with values 0.37 and the residental area was 1.07 which meant average. There were found 4 and 5 flies species at IWMS Piyungan and residental area. The flies species that were found are, Musca domestica, Fannia sp., Chrysomya megacephala, Lucilia sp., and Sarcophaga sp. The distribution pattern of each species was classified into clumped. The distribution pattern was determine by factors like temperature that were about 28 until 29.5 degrees Celcius, and humidity were around 69 until 78 percents. Keywords: Density, Diversity, Flies, IWMS Piyungan, Vector Share Link | Plain Format | Corresponding Author (Dila Hening Windyaraini)

18	Biology	ABS-409
THE CHARACTERISTICS OF DIATOM IN DIFFERENT PRESERVATION METHODS: A COMPARISON STUDY Muhammad Hadi El Amin(a), Nining Betawati Prihantini (a*) a) Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, 16424, Depok, West Java, Indonesia *Corresponding author: nining[at]sci.ui.ac.id Abstract Diatoms are one kind of microalgae which often used as a bioindicator of water quality because of their cosmopolite presence and they are easy to recognise. Their characteristics are unique caused of their cells are covered by silica which is called a frustule. Diatom identification is carried out using morphological features that are contained in the frustules. There are some methods for diatoms preservation including using Lugol^s iodine, ethanol and formaldehyde solution. This study aims to compare those solutions. Diatom samples are taken at Indonesia university lake (Situ Agathis). Those solutions had prepared with one control and four observation. Lugol^s iodine was prepared with a dose of 1%. Ethanol was used at doses of 20% while the formaldehyde solution was used at 4%. The observed diatom characteristics include the presence of cytoplasm, and ornamentation of the diatom frustules (including striae and raphes) were observed to find the distinction in those methods. the presence of cytoplasm in each cell was not too different in the treatment, with the percentage of cytoplasmic cells being 73% (ethanol and formalin) while in Lugol the percentage of cytoplasmic cells was 65% and in the control was 76%. Based on the results, it was found that there was no significant effect of differences in preservation methods on cells with the same cytoplasm or not with a p value > 0.05 Keywords: Diatom, Preservation method, Characteristics Share Link | Plain Format | Corresponding Author (Muhammad Hadi El Amin)

19	Biology	ABS-184
The Flower Bud Development and Pollen Ultrastructure of Ciplukan (Physalis angulata L.) Utaminingsih (a), Tiara Suryani (b), Siti Susanti (c), Maryani (d*) (a), (b), (c), (*d) Plant Structure and Development Laboratory, Faculty of Biology, Universitas Gadjah Mada- Jl. Teknika Selatan, Sekip Utara, Yogyakarta 55281 *mmyani[at]ugm.ac.id Abstract The Solanaceae family is a group of plants that have many taxa with high economic value. The Physalis genus is one of the members of the family Solanaceae which is widely used in the medical field, as well as food ingredients. The concentration of secondary metabolites in Physalis increases with the development of flower buds, so data is needed regarding the development of flower buds. P. angulata and P. divaricata D. Don are two species that have many similarities. The purpose of this study was to determine the development of P. angulata L. flower buds and to compare the flower morphological characteristics of some species from the same family and to compare the pollen ultrastructure of P. angulata L. and P. divaricata D.Don. The youngest buds and buds at anthesis had a diameter of 1.1 ± 0.02 mm and 9.3 ± 0.67 mm and a length of 2.4 ± 0.02 mm and 9.3 ± 4.67 mm respectively. The easiest flower morphological character to distinguish between genus is the color of petal. Pollen ultrastructures P. divaricata and P. angulata have a prolate shape, tricolpate type aperture, and verrucate ornamentation. Keywords: Solanaceae, Physalis, flower bud, pollen ultrastructure Share Link | Plain Format | Corresponding Author (Utaminingsih -)

20	Biology	ABS-469
Secretory Structures, Histochemistry, and Antibacterial Activity of Macaranga gigantea as a Medicinal Plant Evi Muliyah (a*), Yohana C Sulistyaningsih (b), Sulistijorini (b), Mohamad Rafi (c) a) Department of Biology Education, Faculty of Tarbiya and Teaching Sciences, Universitas Islam Negeri Syarif Hidayatullah Jakarta, Banten 15412, Indonesia *evimuliyah[at]uinjkt.ac.id b) Department of Biology, Faculty of Mathematics and Natural Sciences, IPB University, Bogor 16680, Indonesia c) Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Bogor 16680, Indonesia Abstract Macaranga gigantea is a member of the family Euphorbiaceae. Anak Dalam tribe, an indigenous people that live in Bukit Duabelas National Park Jambi province used to utilize the bark of M. gigantea to treat diarrhea. This study aimed to identify the secretory structure, histochemical aspects, and antibacterial potency of M. gigantea bark. The type of secretory structure was carried out. M. gigantea bark has idioblast cells as a secretory structure. The idioblast cells are distributed in the cortex. The idioblast cells contain phenolic compounds, alkaloids, and terpenoids. The well diffusion method was used to test different concentrations of bark extracts. According to the findings, M. gigantea bark extract at 100 mg/mL had the best inhibitory activity against Staphylococcus aureus and Escherichia coli, with a maximal inhibition zone. Keywords: antibacterial activity- diarrhea- histochemistry- idioblast cells- Macaranga gigantea Share Link | Plain Format | Corresponding Author (Evi Muliyah)

21	Biology	ABS-217
Analysis of Macroalgae Diversity In The West Coastal Of Pananjung Beach, Pangandaran Aip M. Irpan(a*), Surti Kurniasih(b), Susi Sutjihati(b), Resmilia Anugrah(b), Siti Aqilah(a), and Muhamad Alfieansyah(a) a) Science Education Study Program, Faculty of Education and Teacher Training, Pakuan University, Bogor, Indonesia *aif.irpan[at]unpak.ac.id b) Biology Education study Program, Faculty of Education and Teacher Training, Pakuan University, Bogor, Indonesia Abstract Abstract. The needed information about the diversity data in the area that can be used in ecological areas in the future requested to be investigated. This study aims to analyze the diversity of macroalgae on the west coast of the coast of Pananjung, Pangandaran. The study was conducted from March to April 2020. This research used a survey method using three stations representing the research region. Sampling was taken out in the intertidal zone of the Coastal Coast of West Pananjung, Pangandaran Nature Reserve, West Java using the purposive sampling method. Data taken consisted of macroalgae types found at each observation station and measurements of environmental data consisting of pH, temperature, salinity, and flow velocity. The results showed that there were 21 species of macroalgae grouped into 9 orders, 13 families, and 3 divisions. The research can conclude that macroalgae in Pananjung Barat Beach Pangandaran have an index value that indicates a moderate category, an evenness index classified as a low category, and a dominance index classified as a low category that shows the dominant species was there and it was Padina australis. Keywords: Macroalgae- Diversity- West Pananjung Beach- Pangandaran Nature Reserve Share Link | Plain Format | Corresponding Author (Aip Muhamad Irpan)

22	Biology	ABS-481
Non-Native Plant Species of the Habitat of Fuscous Javan Langur in Mount Merbabu Alanindra Saputra (a) (b)*, Nurmiyati (a) (b), Puguh Karyanto (b) (a) Doctor Biology Programe, Faculty of Biology, Universitas Gadjah Mada, *alanindra[at]staff.uns.ac.id (b) Bachelor of Biology Education, Universitas Sebelas Maret Abstract Habitat degradation and fragmentation can provide opportunities for succession of non-native species. Mount Merbabu in Central Java is one of the protected areas in Java that has experienced forest degradation and fragmentation, threatening the habitat of the rare primate Javan Langur Fuscous Presbytis comata fredericae. Currently, non-native plant species have created a lot of cover on forest soils. This forest land cover can lead to ecological consequences, such as competition for nutrients and space with native plant species. Characteristics of non-native species have the ability to grow fast, reproduce quickly, spread widely, and have a wide range of tolerance to environmental conditions. This will affect the forest ecosystem and affect feed intake for Javan Fuscous Langur. Our research aims to study the kinds of non-native plant species. The method used is a square plot with a size of 5x5m with a point spread by purposive sampling, as many as 100 plots. The results obtained are in the form of a list of non-native plant species and an index of importance. There were 6 non-native plant species found and 25 understory plant species. The index of importance of non-native plant species for overstory plants is quite high, reaching 31% for Acacia decurens, 24% Cupressus arizonica, 21.35% Cupressus lusitanica mill. As for the understory species, the highest important value index is the Ageratina siparia (77.7%) which dominates the area, and Chromolaena odorata (16.97%). This species has the potential to become food for the Javan Lutung Fuscous Presbytis comata fredericae and also a competitor to native plants that affect the stability of the habitat ecosystem of the Javan Lutung Fuscous Presbytis comata fredericae. Keywords: Presbytis comata fredericae, Non-Native Plant Species, Habitat, feed Share Link | Plain Format | Corresponding Author (Alanindra Saputra)

23	Biology Education	ABS-515
Implementation Of Online Programming Software To Explorate The Computational Thinking Ability Of Biological Education Students Sri Maryanti1 (a*), Pina Pitriana,(b), Dede Trie Kurniawan (c) UIN SUNAN GUNUNG DJATI BANDUNG Abstract Abstract. This study aims to explore the Computational Thinking (CT) ability of biology education students in artificial intelligence courses through code.org programming as online programming software. During the process of making media students are required to solve problems systematically and design systems with coding for beginners. This process will be carried out when a biology education student becomes a biology teacher. The research method used in this study is descriptive with a qualitative approach because it is based on observational data from computational media produced in accordance with artificial intelligence learning for education and based on CT indicators. The subjects of this study involved 73 students of the Biology Education Study Program in 2021 with descriptive data analysis techniques. The formulation of the problem in this study reveals how the process of using the code.org platform by students to train computational thinking and how students respond to the use of the code.org platform that has been captured through a questionnaire distributed via google form. The results showed that students were able to follow each stage of making educational game projects through the code.org platform to practice computational thinking skills in accordance with the success of the stages of making simple projects through good, systematic and problem solving coding. Student responses to the code.org platform as an online program tool that can hone computational thinking can provide creativity and enthusiasm for student learning as evidenced by the results of a questionnaire, 63.3% of students strongly agree that this increases creativity. Keywords: Computional Thinking (CT), Online Programming Software, paltform code.org Share Link | Plain Format | Corresponding Author (Sri Maryanti)

24	Biology Education	ABS-519
The Efforts to Improve Students^ Research Skills and Self-Regulated Learning on Environmental Change Materials through Mini-Research-Based Learning with the Scientific Investigation Models Eliya Mei Sisri 1* , Hertien Koosbandiah Surtikanti 2, Rini Solihat2 12Post-Biology Education Study Program, Universitas Pendidikan Indonesia, Jl. Dr. Setiabudhi No. 229, Bandung 40154, Indonesia Abstract Equipping students with research skills and self-regulated learning is considered very important to face the challenges of the 21st century, so that innovation and adjustment are needed in the learning process. The aim of this study is to examine the application of mini research-based learning with a scientific investigation model on students^ research skills and self-regulated learning. The research method used is a quasi-experimental design with the matching-only pretest-posttest control group design, the sample is taken by purposive sampling. The population of this research is class X students in one of the public high schools in Rokan Hulu district, Riau Province. The sample consisted of one experimental group class and one control group class. The learning material used is environmental change. Data collection instruments used research skills tests, Motivated Strategies for Learning Questionnaire (MSLQ) and science investigation rubrics. The pretest and posttest data were analyzed to obtain the N-gain of students^ research skills and self-regulated learning. From the results of this study, it is known that the improvement in research skills of the experimental group is 30% in the high category and 70% in the moderate category. The improvement of research skills in the control group is 50% in the moderate category and 50% in the low category.Thus, the results of this study indicate that there is a positive contribution from the application of mini research-based learning with the scientific investigation model in improving students^ research skills and self-regulated learning. Keywords: Mini research learning with scientific investigation model, Research skills, Self-regulated learning, Environmental change Share Link | Plain Format | Corresponding Author (Eliya Mei Sisri)

25	Biology Education	ABS-265
Enhancing Conceptual Understanding and Environmental Literacy Using Online Learning Modules on Ecological Systems Geminna Wel C. Del Rosario and Maricar S. Prudente Department of Science Education, BAGCED, De La Salle University, Manila Abstract The threats of environmental problems necessitate urgent attention and action. To solve these problems, the inclusion of Environmental Literacy is recognized as an essential part of the curriculum in Science. Moreover, schools are mandated to be innovative and resourceful in delivering quality, accessible, and relevant education amidst COVID-19. With this, the researchers developed, implemented, and examined the use of Online Learning Modules in an effort to improve the teaching of ecological systems, thereby improving the students^ conceptual understanding and their environmental literacy. The Online Learning Modules on Ecological Systems (OLMES) was designed, developed, and validated for teaching Science to Grade 6 students (n=60). Results revealed that students^ conceptual understanding on ecological systems significantly changed after the exposure to OLMES (t = 21.81) with a large effect size (d=3.07). Improvement on students^ environmental literacy was also evident after the implementation of the study. This is supported by the increased scores on the different variables on environmental literacy survey (ecological knowledge t=12.49, verbal commitment t=5.38, environmental sensitivity t=2.55, environmental feelings t= 3.82, issue analysis and action planning t=13.11, and actual commitment t=4.45). Classroom observations, and students^ responses during interviews and in their reflection logs also showed that the conceptual understanding and environmental literacy of the students had significantly improved after the OLMES implementation. Overall, the evidences provided in this study imply that the use of OLMES contributed to the improvement of teaching practices which resulted to better conceptual understanding and improved environmental literacy among the student participants. Keywords: environmental literacy, ecological systems, science education, online distance learning, online learning module, conceptual understanding Share Link | Plain Format | Corresponding Author (Geminna Wel Del Rosario)

26	Biology Education	ABS-272
Cognitive Activity: How It Affected by Visual Reading Strategies and MbL for Students Learning Benefit? Aisyah Zumira (a*), Rini Solihat (b) a) Biology Education, School of Postgraduates, Universitas Pendidikan Indonesia, Bandung, Indonesia *aisyahzumira[at]gmail.com b) Biology Education Department, Faculty of Mathematics and Science Education, Universitas Pendidikan Indonesia, Bandung, Indonesia Abstract Many of biology lessons content is abstract. Pictures, diagrams, and models are some examples of visual representations used by teachers as teaching strategies. Representations facilitate both of teacher and students in learning process. For teacher, representations help teacher in knowledge transfer process. As for students, representation makes it easier for them to understand the content and reduces the possibility of misconceptions. Several studies show that students^ visual representation skills are still very low. The ability of visual representation can be seen from the cognitive activity that appears in the process of understanding the information in the form of a given visualization. The low ability of students^ visual representation is influenced by the lack of use of visual reading strategies which also affect students^ cognitive activities. Another thing that can affect students^ cognitive activity is the use of modeling based learning (MbL). Then, this study purpose was to determine the effect and relationship between visual reading strategies and MbL on cognitive activities in biology learning process. There are fifteen scientific article from the last ten years publications used in this study from credible journal in biology education. The results of this review indicate that visual reading strategies and the use of MbL effect on students^ cognitive activities when studying biology learning content. For example, reduced students^ misconceptions after the use of visual representations and MbL. Therefore, the results of this review can be an input for biology teachers to improve the learning process and maximize students^ learning outcomes in the future. Keywords: Cognitive activity- visual representations- modeling based learning- biology learning process Share Link | Plain Format | Corresponding Author (Aisyah Zumira)

27	Biology Education	ABS-26
Teacher and students expectation towards biochemistry STEM based module in promoting critical thinking and creativity N Eurika (a*) (b), Suratno (a), Sutarto (a), J Prihatin (a) a) Science Education Doctoral Program, Universitas Jember, Jl. Kalimantan No. 37, Jember 68121, Indonesia *eurika[at]unmuhjember.ac.id b) Biology Education Program, Universitas Muhammadiyah Jember, Jl. Karimata No. 49, Jember 68121, Indonesia Abstract The aim of this research is to know the expectations of teachers and students towards STEM-based biochemistry module in promoting critical thinking and creativity. Many complex problems in the real world today involve higher-order thinking skills, especially critical and creative thinking skills for problem-solving and decision-making in all aspects of life. An interdisciplinary approach and appropriate learning module involving elements of science, technology, engineering, and mathematics (STEM) integrated into the engineering design process (EDP) are deemed to be able to improve student learning experiences through problem-solving activities in the real world. This study used a qualitative descriptive method through a survey. Subject are teachers of biochemistry course, as well as students who have taken biochemistry courses and fill the survey voluntarily. Research data were collected through questionnaires and analyzed using descriptive analysis. The results showed that that the expectations of teachers and students towards STEM-based Biochemistry modules to develop critical thinking skills and creativity students emphasize on aspects design, content, and presentations that are aligned with STEM characteristics. The results of this research will be a reference in the process of developing STEM-based Biochemistry teaching materials that are able to develop critical thinking skills and creativity of students. Keywords: Teacher expectation, students expectation, biochemistry STEM module Share Link | Plain Format | Corresponding Author (Novy Eurika)

28	Biology Education	ABS-301
Development of Digital Modules in the Environmental Toxicology course at the Undergraduate Level at Biology Program Study Hertien Koosbandiah Surtikanti (a*), Tri Suwandi (b), Kurnia Utami (c) and Chika Putri Faritzah (c) a) Program Studi Biologi, FPMIPA UPI Jalan Dr. Setiabudi 229 Bandung. b) Program Studi Pendidikan Biologi FPMIPA UPI Jalan Dr. Setiabudi 229 Bandung c) Program Studi Pasca Pendidikan Biologi FPMIPA UPI Jalan Dr. Setiabudi 229 Bandung Abstract The need for digital modules is required in the implementation of distance learning. This study aims to develop a digital module in Environmental Toxicology lectures at the undergraduate level of the Faculty of Mathematics and Natural Sciences Education. The existence of this digital module can support the implementation of the Independent Learning Program - Independent Campus (MBKM) in the FPMIPA. This development is carried out through the ADDIE approach which consists of 5 stages, namely analysis, design, development, implementation and evaluation. This study involved two experts as digital module validators and 10 students who were involved in learning Environmental Toxicology through Learning Management System (LMS). At the end of the implementation, an evaluation of students will be carried out, to determine the level of student understanding during the online independent learning process. The results of this development are in the form of a digital module consisting of 14 meeting materials, mid term- final exam- content on the SPADA-UPI Learning Management System- and IPR Digital Module Innovation. Based on the results of the validation and readability test, the resulting digital module is feasible and can be used to support online learning of Environmental Toxicology. Keywords: Digital module, environmental toxicology, LMS, ADDIE Share Link | Plain Format | Corresponding Author (Hertien Koosbandiah Surtikanti)

29	Biology Education	ABS-310
Is Metacognitive awareness correlated with collaboration in science class? Case study of ASICC learning model. Poppy Rahmatika Primandiri (a*), Mumun Nurmilawati (a), Sri Panca Setyawati (a), Zainal Afandi (a), Sulistiono (a), Siti Zubaidah (b), Agus Muji Santoso (a) a) Universitas Nusantara PGRI Kediri Jalan KH. Achmad Dahlan 76, Kota Kediri, East Java Indonesia *poppyprimandiri[at]unpkediri.ac.id b) FMIPA, Universitas Negeri Malang Jalan Semarang 5, Kota Malang, East Java, Indonesia Abstract The results of a preliminary study reveal that the ASICC learning model can reduce the collaboration gap between students with different academic abilities. In theory, collaboration requires a strong mental awareness because it is one of form of students to meet learning needs when facing difficulties. This is one of the dimensions of metacognition. Therefore, this study aims to reveal the correlation between collaboration skills and metacognition. The research was carried out in three high schools in East Java by using quasi-experimental research with a non-equivalent pre-post test control group design. Metacognitive data was measured using the Metacognitive Awareness Inventory and collaboration was measured using the Collaboration Awareness Inventory. Both instruments were used at the beginning and at the end of the lesson both in the control class and experimental class (the class used the ASICC learning model). Simple correlation analysis was performed with SPSS after the data was declared homogeneous and normal. This research reveals that there is a positive correlation. However, the correlation between collaboration and metacognition was moderate (r=0,59). Further research is needed to uncover the factors that influence the collaboration of students with different academic abilities. Keywords: ASICC, metacognition, collaboration Share Link | Plain Format | Corresponding Author (Agus Muji Santoso)

30	Biology Education	ABS-311
Biosystematics Learning Design Using Biodiversity of Local Medicinal Plants for Prospective Biology Teachers Agus Muji Santoso (a*), Poppy Rahmatika Primandiri (a), Siti Zubaidah (b) a) Nusantara PGRI Kediri of University Jalan KH. Achmad Dahlan 76, Kota Kediri, East Java Indonesia *agusmujisantoso[at]unpkediri.ac.id b) FMIPA, Universitas Negeri Malang Jalan Semarang 5, Kota Malang, East Java, Indonesia Abstract A preliminary study revealed that prospective Biology teacher students had difficulties in understanding the concept of plant relationships. This is because the learning design still does not use the right context. This study aims to determine the role of the use of context in helping students understand the concept of plant relationships. The context used in this research is various types of local medicinal plants in Kediri. This research was conducted by using design research (validation studies) in the Biosystematics course class. There were three stages of the research: preliminary design, teaching experiment, and retrospective analysis. This study reveals that (1) the context of the types of medicinal plants used in learning should be more than three families- (2) using the plants that can be collected directly by students so that they can be observed and collected in the laboratory- (3) the stage of learning design activities were determining goals, collecting the various herbs, observing samples as OTU (Operating Taxonomy Units), tabulating data, constructing relationship trees with a phenetic approach, interpreting and communicating the results. Keywords: validation studies, design research, medicinal plant, contextual learning Share Link | Plain Format | Corresponding Author (Agus Muji Santoso)

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