In vitro organogenesis induction of potato (Solanum tuberosum L.) stem in MS media with different thidiazuron (TDZ) concentration Jefri Saputro, Yulita Nurchayati, and Nintya Setiari
Departemen Biologi, Universitas Diponegoro, Jl. Prof. Sudarto No. 13, Semarang 50275, Indonesia
Abstract
Potatoes (Solanum tuberosum L.) are a potential source of carbohydrates in food diversification. Conventional propagation of seeds is not effective for national seed stock, so in vitro propagation techniques need to be done. Factors that can spur organogenesis are the types of explants and the use of Thidiazuron (TDZ). This research aims to determine and analyze the types of explants and TDZ concentrations for enhancing potato organogenesis. The method used is induction of organogenesis. Stem explants are cut transversely and initiated in MS media with different TDZ concentrations. The research design used a completely randomized design (CRD) with factorial pattern 2x4. The first factor is explant type and the second factor is TDZ concentration (0, 4.5, 6 and 7.5ppm). The results showed that stem explants with shoot tended to increase the organogenesis induction of potato plants compared to stem explants without shoots. An increase in TDZ concentrations to 4.5ppm tended to increase all parameters researched, except shoot length and root length. It produce the best growth of shoots and leaves. The type of explant and TDZ concentration significantly affect the number of shoots, shoot length, shoot width, shoot emergence, number of leaves, number of roots, root length, and root emergence.
Keywords: Solanum tuberosum, TDZ, type of explant, organogenesis, seed propragation
