Extraction and Identification of Carotenoids from The Skin of Banana Kepok (Musa paradisiaca L.) Nunung Kurniasih (a*), Wulan Sari (b), Assyifa Junitasari (c)
a) b) c) Chemisrty Departement, Faculty of Science and Technology, UIN Sunan Gunung Djati Bandung, Jl. A.H. Nasution No. 105, Cipadung, Bandung 40614, Indonesia
Abstract
Banana peel is a waste material or banana waste that is quite large in number. One of The compounds contained in banana peels is carotenoids. Carotenoids have benefits as precursors of vitamin A, antioxidants, immune enhancement, and several groups of carotenoids can be used as a food coloring. This study aims to determine the compound^s secondary metabolites contained in kepok banana peels, determine the best eluent for separation crude extract of carotenoids from kepok banana peels and determine the type of carotenoids present in the skin mashed banana. This research includes extraction which is done by the maceration method using acetone solvent. The carotenoid extract obtained was then partitioned using n-hexane, ethyl acetate, and water. Extracts of n-hexane, ethyl acetate, and water fractions were concentrated and tested for phytochemical screening. Compounds contained in banana peels in the n-hexane fraction are triterpenoids, in the ethyl. fraction Acetate is a flavonoid and the water fraction contains tannins, polyphenols, and flavonoids. In concentrated extract n-hexane, ethyl acetate, and water fractions were separated from carotenoids by thin-layer chromatography with eluent variations of n-hexane: acetone (6:4), n-hexane: chloroform (8:2), and n-hexane. Next result Thin-layer chromatography was identified using UV-Vis spectrophotometer and FTIR. Results Separation by thin-layer chromatography showed that the carotenoid content was present in the n-hexane fraction with the best eluent n-hexane: acetone (6:4). Results of identification of UV-Vis and FTIR. spectrophotometers showed that the types of carotenoids contained in kepok banana peels were siponaxantin and mitiloxantin
