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31	Chemistry	ABS-513
Analysis of Chromium Content in fish and water at Cisangkuy River and It’s Application on Heuristic Writing Science Worksheet Yulia Sukmawardani, Mia Amelia Rahmawati, Lella Nurlaela, and Neneng Windayani Chemistry Education Study Programme, Faculty of Tarbiya and Teacher Training, UIN Sunan Gunung Djati Bandung, Indonesia Abstract The study in this research is based on the needed for alternative student worksheets of analytical chemistry course on the concept of anion reactions. This research aims to create a student worksheet based SWH (Science Writing Heuristic), determine the feasibility of SWH worksheet, and to describe the characterization results of chromium metal content in fish and water in Cisangkuy river using AAS. SWH worksheet are created by using research Development method. Instruments used in this study include the feasibility test format of student worksheets, questionnaire feasibility test student worksheet, and assessment tools of student worksheets. The validation test result based on SWH student format on chromium metal content analysis in fish and wate in Cisangkuy River has been validated with the average value of rcount obtained at 0.83 and the average feasibility of presentation is 83%, while the concentration of chromium metal content in fish in Cisangkuy river using AAS is 0.0691 mg/L, in the water is 0.2205 mg/L for sample 1 and 0.4871 for sample 2. The concentration is still below the threshold established by Government Regulation of the Republic of Indonesia Number 82 of 2001, the maximum allowable level for chromium metal (Cr) is 0.5 mg/L. Keywords: Fish; SWH worksheet; chromium; Cisangkuy river; AAS Share Link | Plain Format | Corresponding Author (Yulia Sukmawardani)

32	Chemistry	ABS-514
Utilization of Wood sawdust as Heavy Metal Adsorbent in the Waste Processing Industry of Paint Yulia Sukmawardani, Pitriani, Pina Pitriana, and Neneng Windayani Chemistry Education Study Programme, Faculty of Tarbiya and Teacher Training, UIN Sunan Gunung Djati Bandung Abstract This study aims to utilize wood sawdust waste in the paint industry waste treatment. The first thing to do is a qualitative analysis of heavy metals before and after the processing. Furthermore, the parameters measured are temperature, pH, TDS, and dissolved oxygen (Dislove Oxgen). The use of wood sawdust as a heavy metal adsorbent in paint industry wastes is able to provide efficient results with no sediment formation when conducting qualitative analysis of Cu2+ and Cd2+ metal ions and raising the waste parameters to be environmentally friendly. This shows that the adsorption process works optimally. Keywords: waste, heavy metals, adsorbent, wood sawdust Share Link | Plain Format | Corresponding Author (Yulia Sukmawardani)

33	Chemistry	ABS-516
Utilization of E-module in Polymer Synthetic based on Green Chemistry to Improve Student High Level Thinking Desi Dwi Astuti, Saepudin Rahmatullah, Yulia Sukmawardani, Cucu Zenab Subarkah Chemistry Education Study Programme, Faculty of Tarbiya and Teacher Training, UIN Sunan Gunung Djati Bandung Abstract Increased thinking at a high level is needed so that students are able to solve the problems faced in their lives based on Islamic values. This study aims to analyze the improvement of higher order thinking skills based on Islamic values on the application of green chemistry-based synthetic polymer e-modules. The research design used was a one-group pretest and posttest with research subjects that were 26 semester VI students of the Chemistry Education Study Program. The results of this study indicate that the application of synthetic chemistry based on green chemistry e-module can improve the ability of high-level thinking based on Islamic values based on the average gain of n-gain 0.647 which is included in the medium category. Learning using this e-module can improve learning outcomes by making learning situations more active so as to better understand the concept of synthetic polymers based on green chemistry. Thus, the application of synthetic chemistry based on green chemistry e-module can improve the ability to think at a high level. Keywords: high thinking level, e-module, polymer synthetic Share Link | Plain Format | Corresponding Author (Yulia Sukmawardani)

34	Chemistry	ABS-262
Antioxidant Activity of Potassium Aluminum Sulphate (Alum) as Medicine Preparation for Vaginal Discharge Titik Taufikurohmah(a*), Fadlilah Gita Shalli(a), Eka Apriyosa(b) and Suyatno(a) (a) Departemen of Chemistry, Faculty of Mathematics and Natural Sciences, State University of Surabaya (UNESA), Indonesia *Corresponding author : titiktaufikurohmah[at]unesa.ac.id (2) PT. Khanza Ekselensia Utama, Indonesia Abstract Potassium aluminium sulphate (Alum), KAl(SO4)2.12H2O, is an inorganic salt with crystalline form. Alum has antioxidant activity that can play a role in treatment of excessive vaginal discharge. Excessive vaginal discharge can triggering an oxidative stress which marked by existance of reactive oxygen species (ROS), so that cause a cell death and tissue damage. This study aims to know the antioxidant activity of alum that can used as medicine preparation for vaginal discharge. The method used is the DPPH with concentration 0.003% and variation concentration of alum is 2; 1; 0.5; and 0.25 (%w/v). Then reduction at intervals of 30, 60, and 90 minutes and measured the absorbance using a UV-Vis Spectrophotometer at wavelength of 516nm. Inhibition percents and antioxidant activity determined through IC50 value. Result in this study showed high inhibition percent at high concentration. At time intervals of 30, 60, and 90 minutes reduction of DPPH showed the IC50 values that increased significantly of 5.2; 7; and 7.9. Based on the results can concluded that the higher concentration of alum the higher percent inhibition obtained and longer interaction between alum with free radicals cause decrease antioxidant activity proved by increasing IC50 value. Keywords: Potassium Alum; Antioxidant; IC50; Vaginal Discharge; Medicine Share Link | Plain Format | Corresponding Author (Fadlilah Gita Shalli)

35	Chemistry	ABS-7
Quality Evaluation for Chili Hybrid Variety Candidate Cultivated in Highland Rahayu, ST., Kirana, R., Levianny, PS Indonesian Vegetable Research Institute Tangkuban Perahu Street 517, Lembang, West Java Abstract Chili is one of potential vegetable due to its high demand and as an important source of nutrition. Hybrid chili has several advantages so it is beneficial to be cultivated. The purpose of this study is to examine the quality of several prospective varieties of large chili hybrids which will be released as new varieties. This research was was conducted in Indonesian Vegetable Research Institute in 2019 at the Post Harvest Physiology Laboratory, West Bandung Regency, West Java. The materials used in the study were 3 chili variety candidates and 2 existing varieties that harvested in Lembang during rainy season. The study used randomized block design with 5 treatments and 5 replications. Physical parameters observed were weight, diameter, length, and texture. Chemical parameters includes water content (gravimetric method), Total Soluble Solid (TSS), vitamin C (titration method), and ash content. Organoleptic test was carried out by 15 untrained panelists using scale from of 1 (like extremely) to 5 (dislike extremely) in order to determine consumer acceptance of the freshness of chillies. The organoleptic test was conducted on days 0, 4, and 7. The results showed Genotype No. 4 had weight, diameter and vitamin C close to the comparative varieties. Genotypes no. 2 and 3 have quality parameters (color, shape, size, appearance, and freshness) preferred by consumers during storage until the 7th day at room temperature in Lembang area of West Java. Keywords: Chili, evaluation, hybrid, quality Share Link | Plain Format | Corresponding Author (SUWARNI TRI RAHAYU)

36	Chemistry	ABS-519
A NEW D-LOOP MITOCHONDRIAL NUCLEOTIDE VARIATION FROM INDIVIDU AT NAGA AND KUTA TRADITIONAL VILLAGE Tina Dewi Rosahdi (a*), Rina Budi Satiyarti (b), Dini Dwi Nurbaeti (a) a. Jurusan Kimia, Fakultas Sains dan Teknologi, UIN Sunan Gunung Djati Bandung *tina_dr[at]uinsgd.ac.id b. Jurusan Pendidikan Biologi, Fakultas Tarbiyah dan Keguruan, UIN Raden Inten Lampung Abstract The D-Loop region of human mitochondrial DNA (mtDNA) has a higher level of polymorphism compared to nuclear DNA. Therefore, the mtDNA D-Loop area can determine a particular individual or ethnic identity. This study aims to determine the variation of the D-Loop region nucleotide sequence, namely the Hipervariable I (HV1) region in normal tribal individuals in Kampung Naga and Kampung Kuta. The sample used is the hair root. In determining the variation of nucleotides has been carried out a series of stages of research, namely the isolation of mitochondrial DNA from hair root samples using buffer lysis, mtDNA sample amplification using PCR techniques with M1 primers and HV2R primers, detection of D-Loop region mtDNA fragments by agarose gel electrophoresis, and sequencing of PCR products by the Sanger Dideoxy method and nucleotide sequence analysis of the sequencing results. The results of DNA amplification by PCR showed a band in the area of 1.0 kb. Homology analysis was carried out by comparing the nucleotide sequences of samples with the Cambridge nucleotide sequence, which shows that there are 10 variations. The new variation (morph) is determined by comparing the results of the variations of the two samples with variations from Homo Sapiens Indonesia including Baduy, Sundanese, Javanese (Sangiran), and Madura. Homology results showed 4 new variations, namely c(16184)A, t(16209)C, a(16272)G, t(16519)C. Sapiens Kuta village and Sundanese (general) have a close genetic relationship. The ancestors of the two Homo Sapiens were closely related to Homo Sapiens Naga, Homo Sapiens Naga are closely related to the Homo Sapiens of the Baduy. The ancestors of Homo sapiens, Kuta, Sundanese, Naga and Baduy, are closely related to Homo sapiens Sangiran (Java). Homo Sapiens Madura is most likely the oldest Homo sapiens seen from phylogenetic trees. The results of this study are expected to be able to add Indonesias normal human database. Keywords: Mitochondrial DNA, D-Loop, polymorphism, PCR Share Link | Plain Format | Corresponding Author (Tina Dewi Rosahdi)

37	Chemistry	ABS-11
Inhibitory effect of tannin extract from Acacia bark (Acacia mangium Willd) against gastro-intestinal pathogenic bacteria Desi M. Sinaga (a*), Erika B. Laconi (b), Anuraga Jayanegara(b) Department of Nutrition and Feed Technology, Faculty of Animal Science, IPB University, Bogor 16680, Indonesia Abstract Acacia (Acacia mangium Willd) is a fast growing tree species that widely found in Indonesia. Acacia bark contains a number of secondary metabolites such as tannin, saponin and flavonoid that may inhibit the growth of undesirable microbes incuding the pathogenic ones. The purpose of this research was to evaluate the antimicrobial activity of tannin extract obtained from Acacia bark against common pathogenic bacteria species present in the gastro-intestinal tract of livestock, i.e., Salmonella typhimurium and Eschericia coli as Gram negative bacteria. The disc diffusion method was employed for assessing the antimicrobial activity of Acacia tannin extract. Treatments consisted of negative control (NC), positive control by using chloramphenicol (PC), Acacia tannin extract at two concentrations, i.e., 1% (AT1) and 2% w/v (AT2), and commercial chestnut tannin at 1% (CT1) and 2% w/v (CT2). Data were analyzed by using analysis of variance and followed by a post-hoc test namely Duncan’s multiple range test. Results showed that the inhibitory diameters formed on the activity assay of AT in the form powder, liquid and CT with concentration of 2% against Salmonella typhimurium bacteria were 17.3, 7 and 16 mm, respectively. Inhibitory diameters of AT powder, AT liquid and CT against Eschericia coli bacteria were 17.3, 0 and 17 mm, respectively. The AT powder resulted in a higher inhibition of both pathogenic bacteria species than that of liquid form (P<0.05), whereas its inhibitory effect was similar to commercial CT. In conclusion, 2% AT can be used to inhibit the growth of Salmonella typhimurium and Eschericia coli bacteria in which its magnitude of inhibition is comparable with commercial tannin extract from chestnut. Keywords: Antibacteria, Acacia, Chestnut, Extract, Tannin Share Link | Plain Format | Corresponding Author (Desi Maria Sinaga)

38	Chemistry	ABS-274
Development of Interactive Multimedia to Improve Students’ Understanding on Carbonyl Compounds Reaction Mechanisms Concept I I Fauzi, N Windayani* and C D D Sundari Department of Chemical Education, UIN Sunan Gunung Djati Bandung, Jl. A.H. Nasution No. 105, Bandung 40614, Indonesia Abstract Student’s understanding of carbonyl compounds reaction mechanisms needs to be improved by using interactive multimedia. In this paper, the development of interactive multimedia on the concept of carbonyl compounds reaction mechanisms is presented. The interactive multimedia was developed using a Design-Based Research (DBR) method, which consists of three stages, i.e. analysis, design, and development. Expert validation and feasibility test on the interactive multimedia were also carried out. The overall validation test results obtained rcount of 0,83 and the feasibility test results obtained the feasibility percentage of 88,1%. The interactive multimedia was stated as valid and feasible to be used as a learning media. A group of students said that the interactive multimedia could improve their motivation in learning reaction mechanisms. Keywords: carbonyl compound, chemical representation, interactive multimedia, reaction mechanism Share Link | Plain Format | Corresponding Author (Neneng Windayani)

39	Chemistry	ABS-24
The use of organic complexants as probe for toxic metals analysis in environment with digital-image colorimetry M. Lutfi Firdaus, Juwita Megarani, Hadi Apriyoanda, Agus Sundaryono, Eko Swistoro University of Bengkulu, Indonesia Abstract The concern on environmental pollution that currently occurs is toxic heavy metal pollution generated from various industrial activities and daily household activities. To monitor and determine the quantity of pollutants, instruments such as AAS or ICP-MS which are very expensive are needed. Analysis of trace level of heavy metals in polluted environments is a challenging task. First problem is the lack of representative instruments such as ICP-Ms in most Indonesian research institutions due to its expensive price and maintenance. Second problem is extremely low concentration of analytes needs a sophisticated method that is still scarce. This study aims to find a simple method, economical dan portable but also accurate and precise in the analysis of heavy metals. Method which will be used based on colorimetric analysis which is part of spectroscopy. The metal analyzed will be complexed first using an organic complexion such as 1,5-diphenilcarbazide (DPC), alizarin red, dithizone, etc. The colored complex solution is then measured using spectroscopy in the range of visible light region. This study used digital image colorimetry as an alternative that is more economical, practical and environmentally friendly. Image data will be taken using cellphone camera and processed by the primary color system or RGB. The accuracy of this method was compared with UV-Vis spectrophotometry. The results of this research were implemented to students to their understanding on the topic of environmental chemistry. Keywords: Heavy Metals, organic complexants, visible spectrophotometry, digital image. Share Link | Plain Format | Corresponding Author (M. Lutfi Firdaus)

40	Chemistry	ABS-29
Effects of Mycorrhiza Biofertilizer on Anthocyanin Contents and Yield of Various Red Rice Genotypes under Aerobic Irrigation Systems Wayan Wangiyana (a*), I Gusti Putu Muliarta Aryana (a), Ni Wayan Dwiani Dulur (b) (a) Post-Graduate Program, University of Mataram. (*) email: w.wangiyana[at]unram.ac.id (b) Department of Soil Science, Faculty of Agriculture, University of Mataram Abstract Red color in red rice kernels is because of anthocyanins, making red rice to have high health values. Mycorrhizas are reported to affect anthocyanin contents of other crops. This research aimed to examine the effects of mycorrhiza biofertilizer on grain anthocyanin contents and yield of various promising lines of red rice under aerobic irrigation systems, by conducting field experiment in Beleke, West Lombok, Indonesia, from March to July 2018, in Split Plot design with three blocks and two treatment factors, namely red rice genotypes as main plots (G04, G10, G15, G21) and AMF bio-fertilizer as sub-plots (M0= without, M1= with biofertilizer application). Results of data analysis using ANOVA and Tukey’s HSD test indicated that anthocyanin contents and almost all yield components were significantly higher on red rice supplied with AMF biofertilizer than without AMF, while those variables were mostly non-significantly different between red rice genotypes, except for 100 grain weight. Despite no interaction effects, there was a significantly positive correlation between anthocyanin contents and harvest biomass weight on red rice fertilized with AMF biofertilizers, but not on those receiving no AMF, which indicates positive effects of AMF in increasing harvest biomass and grain anthocyanins of those red rice genotypes. Keywords: Anthocyanins. Biofertilizer, Mycorrhiza, Red rice, Aerobic irrigation systems Share Link | Plain Format | Corresponding Author (Wayan Wangiyana)

41	Chemistry	ABS-552
Developing Renewable Thermo-Hydrochromic Bioinorganic Materials from Bone Wastes of Slaughterhouses I Wayan Karyasa Department of Chemistry, Faculty of Maths and Natural Sciences, Universitas Pendidikan Ganesha Abstract Adding value of slaugtherhouses products and wastes today become greater attention, especially bone wastes for producing renewable biomaterials, in supporting sustainable green industries. However, the high potency of developing the renewable bioinorganic materials from bone wastes needs an comprehensive need assesment. The need assesment was conducted through three steps: defining the needs by literature searching and metaanalysis, collecting and analysing data, and making decision of the needs. Results of the study showed that (1) there were prospective and sustainable raw materials from slaughterhouses in Indonesia, especially bones, for developing phosphate based bioinorganic materials; (2) there were some needs of developing bioinorganic nanoparticles materials in M-Si-P-O, M-Al-P-O, and M-Al-Si-P-O systems (with M = main and/or transition metals) for artifisial bones and teeth purposes; and (3) there were some needs of developing thermo-hydrochromic bioinorganic materials sensoric temperature and humidity for supporting artifisial insemination as well as medicinal purposes. The last result of the study was supported by current finding on natural inorganic thermo-hydrochromic pigment. Keywords: bone, thermo-hydrochromic, bioinorganic materials Share Link | Plain Format | Corresponding Author (I Wayan Karyasa)

42	Chemistry	ABS-1067
RGB-ColorMeter on Smartphones as Simple Spectrophotometer: an Alternative Way to Learn Spectrophotometry at the Vocational High School of Chemical Analyst Maria Paristiowati (a*), Moersilah (a), Muktiningsih (a), Annisa Nur Fitria (a) a) Department of Chemistry Education, Universitas Negeri Jakarta Jl. Rawamangun Muka Jakarta 13220, Indonesia *) maria.paristiowati[at]unj.ac.id Abstract This study aims to improve students understanding of the Vocational High School of Analyst Chemistry on spectrophotometric concepts through experiments using smart phone technology. The ColorMeter application with matching red green blue (RGB) colors is used as a simple spectrophotometry. The use of smart phone technology as a simple spectrophotometry in practicum can maximize spectrophotometric learning activities that are often constrained by the limitations of the tool, so that learning objectives can be achieved properly. This RGB–ColorMeter application can be used to read the transmittance of a colored solution by utilizing the complementary colors of the solution being tested. Transmittance data obtained is used to find the absorbance of the solution, so that it can determine the concentration of a substance in the solution. The results showed that the application of RGB–ColorMeter can show a linear graph between concentration and absorbance. The whole process is carried out individually by each student starting from the stages of designing, using, and reviewing this simple spectrophotometer. Students understanding of spectrophotometric concepts increases because each student follows the process thoroughly from the beginning to the final stages of the spectrophotometry practicum. So it can be concluded that the application of RGB–ColorMeter can be used as a simple spectrophotometer in the spectrophotometry practicum at the Vocational High School of Analyst Chemistry. Keywords: RGB-ColorMeter, simple spectrophotometer, Vocational High School of Analyst Chemistry Share Link | Plain Format | Corresponding Author (Maria Paristiowati)

43	Chemistry	ABS-303
Using PhET Simulation to Learning the Concept of Acid-Base G.S. Akbar, O.Nuraida, I.Farida, S.Rahmatullah Depertement of Chemistry Education UIN Sunan Gunung Djati Bandung Abstract This study aims to analyze student outcomes after learning the acid-base concept supported by PhET Simulation. The PhET simulation is used to help visualize the sub-microscopic representation of the acid-base solution. The method used is one group pre-test and post-test design with the subject this study consists of thirty high school students in the Bandung area. The research instrument used was a test that measured the ability to distinguish the strength of acid-base at the level of sub-microscopic representation, calculate the degree of ionization, the relationship of Ka to concentration, and analyze the pH of the solution. The result of this study it can be concluded that the PhET simulation can improve student learning outcomes to the level sub-microscopic representation. Keywords: PhET Simulation, Chemistry Learning, Acid-base Concept Share Link | Plain Format | Corresponding Author (Ida Farida)

44	Chemistry	ABS-816
Integration Dilemmas Stories in STEAM Project of Colloid Endang Natalya, Yuli Rahmawati, and Erdawati Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Indonesia Abstract The study aimed is to develop students’ chemical literacy through dilemmas stories and STEAM project in chemistry learning in colloid topic. The dilemmas stories of environmental pollution were explored to engage students in contextual problems. The STEAM project of smoke absorbance was used as the problem solutions in dilemmas stories. The learning approach consists of several steps such as value reflection, problem solving, project development, project monitoring and evaluation, and transformation. The research employed qualitative approach with data collection of students’ interviews, reflective journals, observations, and chemical literacy tests. Chemical literacy consists of several dimension such as scientific ideas, chemical characteristics, chemistry in context, high-level learning skills, and affective aspects. The results showed that the average chemical literacy ability of students were in the good category. The teacher faced the challenges in developing dilemmas stories to be relevant to chemistry concepts and curricula. Students need to shift their paradigms within active learning approach. The results of this study can be concluded that the integration of dilemmas stories in the STEAM project provides opportunity to develop students chemical literacy. Keywords: Chemistry Learning, Chemistry Literacy, Dilemmas Stories, STEAM Project, Colloid Topic Share Link | Plain Format | Corresponding Author (Endang Natalya)

45	Chemistry	ABS-564
Appropriate Technology in Producing Nila Nyat-nyat (Oreochromicus niloticus): Research on the Effect of Differences in Cooking Time with the Quality of Nila Nyat-Nyat I GDE SURANAYA PANDIT1,2, PANDE AYU NAYA KASIH PERMATANANDA3*, KRSNA YUDHA1 1Department of Fishery and Marine, Faculty of Agriculture, Universitas Warmadewa, Denpasar 80235 2Postgraduate Program, Universitas Warmadewa, Denpasar 80235 3Department of Pharmacology and Pharmacy, Faculty of Medicine and Health Science, Universitas Warmadewa, Denpasar 80235 4Department of Fishery and Marine, Faculty of Agriculture, Universitas Warmadewa, Denpasar 80235 Abstract The right time for cooking nila nyat-nyat is very influential on subjective and objective value of nila nyat-nyat. The goal of this study was to determine the best time in cooking tilapia in order to get the best quality of nila nyat-nyat. The research was conducted at the Laboratory of the Faculty of Agriculture, Warmadewa University and the Faculty of Food Technology, Udayana University. This research used experimental method with completely randomized design of single factors with 6 different treatments and 4 replications. Quality assessment includes subjective tests through organoleptic analysis such as appearance, texture, odor, and taste, and also objective tests or nutritional analysis, such as water, fat, protein, and ash content. Statistical analysis with anova showed that the difference in cooking time gave significant influence in the organoleptic quality and nutritional value of tilapia (p value < 0.01). From this research, we conclude the best time for cooking tilapia was 30 minutes. Keywords: cooking time, nila nyat-nyat, nutritional value, organoleptic quality, tilapia Share Link | Plain Format | Corresponding Author (Suranaya Pandit I Gede)

46	Chemistry	ABS-1077
Force to slice, shelf life, sensory analysis and production process of Tempe Mlanding in Yogyakarta and Bogor Varieties Rahmi Dzulhijjah, Budi Setiawan, Eny Palupi IPB University Abstract Tempe mlanding is a traditional tempe from Gunung Kidul, Yogyakarta Indonesia which is derived from lamtoro seeds and fermented for 48 hours. This article seeks to compare force to slice, shelf life, sensory analysis of tempe mlanding in Yogyakarta and Bogor varieties and provides a profile in traditionally making tempe mlanding in Gunung Kidul, Yogyakarta. The results of the analysis showed that the slice of soybean tempe as a control, tempe mlanding native varieties of Yogyakarta and Bogor varieties decreased with increasing thickness of tempe. Comparison force to slice of soybean tempe (32.16 ± 1.66), tempe mlanding from Yogyakarta (29.25 ± 2.08) and Bogor (32.25 ± 0.66). Based on sensory analysis of raw tempe mlanding, fried, roasted and oven overall have the same level of preference. The shelf life of tempe mlanding both varieties at room temperature reaches 2 days, while at low temperatures it reaches 3 days. The making of tempe mlanding traditionally begins with a day of soaking (24 hours), the process of separating the skin using a stamped foot of the craftsman (diidak-idak), boiling, filtering using Kalo, inoculation process and the fermentation process which is stored in a Tenggok for 48 hours. Keywords: Force to slice, lamtoro seeds, sensory analysis, shelf life, tempe mlanding, Yogyakarta Indonesia Share Link | Plain Format | Corresponding Author (Rahmi Dzulhijjah)

47	Chemistry	ABS-574
Ethanol and Methanol Levels of Red Dragon Fruit Wine (Hylocereus costaricensis) with the treatment of Sugar and Fermentation Time I Wayan Sudiarta, I Wayan Rudi Saputra, Ni Made Ayu Suardani Singapurwa, I Putu Candra, A.A. Made Semariyani Department of Food Science and Technology, Warmadewa University Abstract Red dragon fruit (Hylocereus plyrhizus) contains anthocyanin which is a polyphenol compound that is rich in pigments, determining the formation of red, purple and blue colors of various fruits and vegetables. Wine fermentation is the process of breaking down sugar into alcohol and CO2 gas due to enzymes produced by yeast cells. Factors that influence the fermentation process are the type of yeast, sugar content, nutrition, temperature, aeration and pH. The fermentation time in making fruit wine is very important to know to produce wine with good characteristics. This study aims to determine the effect of sugar levels, and the best fermentation time on the characteristics of red dragon fruit wine. This research was conducted at the Food Processing Laboratory, University of Warmadewa. Research with a completely randomized design consisting of two factors and two replications, the first factor is sugar content. which consists of four levels: 10%, 15%, 20%, 25%. The second factor is fermentation time, which consists of three levels: 10 days, 15 days, 20 days. Variables observed in dragon fruit wine include objective observations: methanol content and ethanol content. From the research results obtained 10.42% ethanol content, and the wine produced does not contain methanol. Keywords: red dragon fruit wine, sugar content, fermentation Share Link | Plain Format | Corresponding Author (I Wayan Sudiarta)

48	Chemistry	ABS-64
Morphological Characteristics, Chemical and Amino Acids Composition of Flours from Velvet Beans Tempe (Mucuna pruriens), an Indigeneous Legumes from Yogyakarta Holif Fitriyah (a*), Faisal Anwar (a), and Eny Palupi (a) (a) Community Nutrition Department, Faculty of Human Ecology, IPB University, Jl. Raya Dramaga, Kampus IPB Dramaga Bogor 16680 West Java, Indonesia *Corresponding author: holiffitria51ipb[at]gmail.com Abstract Velvet beans (Mucuna pruriens) an under-utilized legume which grown predominantly in tropical area, including in Indonesia. The morphologicall characteristics, chemical and amino acid composition of seeds, fermented velvet beans (Benguk tempeh), and Benguk tempeh flours from the legumes Mucuna pruriens was evaluated to determine their potential practical applications. The seeds of velvet beans contains 12.73±0.10, 4.34±0.33, 25.49±0.06, 3.66±0.03, 3.90±0.06, and 53.79±0.26 (% wb) of moisture, fat, crude protein, ash, crude fiber and carbohydrate, respectively. The mineral content of velvet seeds was 76.38±8.77, 6.64±0.67, and 3.08±0.12 mg 100 g-1 of calcium, iron, and zinc, respectively. Fermentation process on velvet beans into tempeh brings nutritional changes including the breakdown of certain nutrition content and reduction of anti-nutritional compounds. The velvet beans tempeh contains 54.76±0.24, 2.40±0.16, 12.84±0.56, 1.02±0.13, 3.66±0.49, and 28.99±0.60 (% wb) moisture, fat, crude protein, ash, crude fiber and carbohydrate, respectively. The mineral content of velvet beans tempeh was 55.49±13.91, 6.56±0.39 and 2.60±0.33 mg 100 g-1 of calcium, iron, and zinc, respectively. The flours were prepared by steam blanching, oven‐drying (60°C) for 7h and milling. The flours contains 13.13±0.52, 6.78±0.72, 25.29±0.41, 0.76±0.04, 4.34±0.32, and 54.06±0.47 (% wb) of moisture, fat, crude protein, ash, crude fiber and carbohydrate, respectively. The mineral content of flour was 29.43±0.00, 5.87±1.36 and 2.56±0.04 mg 100 g-1 of calcium, iron, and zinc, respectively. The essential amino acids profile of total flour proteins compared avourably with the FAO/WHO reference pattern except for deficiency of sulfo amino acids (methionine and sistin) with concentration of 9.72 mg g-1 crude protein. The flours have potential as flour alternatives for food product development. Keywords: Amino acid composition, mineral, proximate, velvet beans Share Link | Plain Format | Corresponding Author (Holif Fitriyah)

49	Chemistry	ABS-1103
Micro Capillary Columns Packed for Determination of Inorganic Ions in Biomaterials Syukrya Ningsih*, Lee Wah Lim, Toyohide Takeuchi 1. Department of Chemistry, Suthan Thaha Saifuddin State Islamic University (UIN), Jambi, Indonesia 2. Department of Chemistry, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193 Abstract Ion Chromatography is widely used analytical technique for determination of anionic and cationic analytes in various sample matrices. Culumns of flexible fused-silica tubing packed with commercially available materials have been prepared. The parameters which affect the chromatographic retention behavior have been examined. For investigation of the optimum concentration of the eluent, the NaCl and KCl concentration was varied from 50 to 25 mM and operated at eluent flow rate 3 µL/min. The microcolumns were prepared in the laboratory from fused silica tubing TSKgel IC-Anion-PW_XL (100 mm long x 0,320 I.D). The developed technique was successfully applied to the simultaneous determination of inorganic anions in real samples. Keywords: micro packed column, ion chromatography, biomaterials Share Link | Plain Format | Corresponding Author (Darma Putra)

50	Chemistry	ABS-848
Assessing the feasibility of chemical laboratory safety videos Ella Fitriani, Zulmanelis Darwis, Lintang Rizkyta Ananda, Anna Rahmadianty, and Maria Paristiowati Universitas Negeri Jakarta, Chemistry Education Department, Faculty of Mathematics and Natural Sciences, Jalan Rawamangun Muka 13220, Jakarta, Indonesia Abstract Learning in a chemistry laboratory has been believed to be able to provide students with more understanding than learning only through textbooks. However, there are many potential hazards to be considered in activities in chemical laboratories. The lack of knowledge and experience of first-year chemistry students causes the possibility of accidents in the chemical laboratory higher. Therefore, chemistry laboratory safety videos were developed and validated by experts. Data was collected using a questionnaire, documentation, and interview. The results of the assessment and expert input are implemented in the developed video. Keywords: Chemical laboratory safety, videos, pre-lab activities Share Link | Plain Format | Corresponding Author (Lintang Rizkyta Ananda)

51	Chemistry	ABS-861
Performance of Neem Seedlings (Azadirachta indica) on Compost and Urea Fertilizer Dose Treatment I Made Aditya Widnyana, Ida Bagus Komang Mahardika*, Made Suarta, Luh Kartini, Made Sri Yuliartini, and Yohanes Parlindungan Situmeang Faculty of Agriculture, Warmadewa University, Denpasar, Indonesia * E-mail: gusmahardika62[at]gmail.com Abstract Neem (Mimba) is one of the types of plants that are the potential to be developed commercially as plantations and community forests. The purpose of this research is to find outperformance of neem seedlings on compost and urea fertilizer dose treatment. This experiment consisted of two factors arranged factorially using a Randomized Block Design. The first factor is the dose of compost with three levels, namely 200 g, 400 g, and 600 g per pot. While the second factor is the dose of urea fertilizer which consists of two levels, namely 2.5 g and 5 g per pot. The results showed that there was an interaction between compost dose and urea dose and had a very significant effect on root length per seed and significantly affected oven-dry weight per seedlings of neem. The highest root length of the neem seedlings of this experiment indicates that the doses of compost at a 200 g per pot and urea fertilizer of 2.5 g per pot was 28.00 cm and oven-dry weight of the roots of neem plant seeds was 2.81 g. Keywords: Compost, urea, neem, Azadirachta indica Share Link | Plain Format | Corresponding Author (Ida Bagus Komang Mahardika)

52	Chemistry	ABS-607
Nutritional Value and Fatty Acids Content of Fried Grasshopper (Valanga nigriconis), an Indigenous Edible Insect from Gunung Kidul Yogyakarta. Sessy Paramita Lirizka(a*), Faisal Anwar(a), Eny palupi(a) a) Community Nutrition Department, Faculty of Human Ecology, IPB University. Jl. Raya Dramaga. Kampus IPB Dramaga Bogor 16680 West Java, Indonesia Abstract Approximately 1,900 insect species are eaten worldwide both in developed and developing countries. Edible insects always been a part of human diets, but in some societies there is a degree of distaste for their consumption. One of edible insects in Indonesia eaten by Yogyakarta people is “belalang kayu” or grasshopper Valanga nigricornis. Chemical parameters observed by proximate analysis and Gas Chromatography for fatty acids. Fried grasshopper contains 5.92% w//w water, 37 fat, 44.8 protein, 4.51 ash, 7.06 (% w/w wb) of moisture, fat, protein, ash and carbohydrate, respectively. The mineral content of fried grasshopper 31.89, 2.79, 3.68 mg/100 g of calcium, zinc, and iron, respectively. Fried grasshopper contains 60.29% total fatty acids and 0.78% (%w/w wb) omega-3 fatty acids. Keywords: edible insect, fatty acids, grasshopper, proximate analysis, Valanga nigricornis Share Link | Plain Format | Corresponding Author (Sessy Paramita Lirizka)

53	Chemistry	ABS-608
Purification of Fim-C-Salmonella typhi Recombinant Protein with Ni-NTA Resin as Raw Material for Typhoid Disease Detection Kit M Nurjayadi1., T Setiyoto1., S F Jinan1., D Hardianto2., A Sulfianti2., K Agustini2., HA El-Enshasy3 1 Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Negeri Jakarta Gedung KH. Hasjim Asj’ari, 6th Floor, Jl. Rawamangun Muka Jakarta Timur, 13220, Indonesia 2 Biofarmaka Research Laboratory LABTIAP BPPT-Serpong, Tangerang Selatan, Banten, Indonesia 3Institute of Bioproduct Development, Universiti Teknologi Malaysia (UTM), Skudai, Johor Bahru, Malaysia Abstract Typhoid caused by Salmonella typhi is an endemic disease in Asia, including Indonesia. Based on data from the World Health Organization (WHO), there are 81% per 100,000 people in Indonesia infected with typhoid. Based on this, development of typhoid detection kit that is simple, fast, specific and sensitive is still needed. One of the developments of detection kits currently available is the antigen and antibody interaction approach. This study aims to obtain comprehensive information about purification of Fim-C-S. typhi recombinant proteins as antigens for raw materials for manufacturing typhoid detection kits. This study uses the IMAC method with Ni2+ metal, which has advantages in high selectivity and purity. The results of this study report that purification of recombinant Fim-C-S. typhi protein inclusion bodies with variations in the binding and washing process at 3, 5, and 7 times gave percent yields correspondingly of 37.84, 39.44, and 38.21% pure protein. Previous studies have also reported variations in binding and washing at 2, 4, and 6 times and gave percent yields respectively were 35.37, 39.11, and 40.49%. Based on the data, we concluded that the variation of 6× is the best repetition variation to get the largest percent yield so that it can be used as a reference in large-scale purification. Keywords: Salmonella typhi, Purification Protein, Recombinant Fim-C S. typhi Protein, IMAC, Ni- NTA Share Link | Plain Format | Corresponding Author (Muktiningsih Nurjayadi)

54	Chemistry	ABS-609
Product Development and Consumers Acceptance of “one bite” Valanga nigricornis Pempek as High Protein Food Suplementation Sessy Paramita Lirizka(a*), Faisal Anwar(a), Eny palupi(a) a) Community Nutrition Department, Faculty of Human Ecology, IPB University. Jl. Raya Dramaga. Kampus IPB Dramaga Bogor 16680 West Java, Indonesia Abstract This research aimed to elevate the value and consumers acceptance of product development of a tasty insect- derived protein product from local indigenous edible insects : “one bite” Valanga nigricornis grasshopper pempek, an Indonesian traditional food. Grasshopper pempek made into small size one bite and was performed in three formulas, from sensory evaluation of three formulas generally showed no significance different (p>0.05), rating test using scale from 1 to 9 (extremely like). From consumers acceptability test, about 83% consumers interested and want to consume “one bite” grasshopper pempek. As many as 93% of consumers choose grasshopper pempek instead of fried grasshopper, one of edible insects-food product in Indonesia. Chemical parameters of pempek observed by proxymat analysis and Gas Chromatography. Valanga nigricornis grasshopper pempek promising nutritious food product high in protein and fatty acids as well as fried grasshopper but grasshopper pempek has higher consumers acceptability. Valanga nigricornis grasshopper may consider as high protein food supplementation to combat protein deficiency in Indonesia. Keywords: consumers acceptance, edible insect , grasshopper, pempek, Valanga nigricornis Share Link | Plain Format | Corresponding Author (Sessy Paramita Lirizka)

55	Chemistry	ABS-617
Variation in Recombinant Protein Production Volume of Fim-C Salmonella typhi as a Raw Material for Typhoid Detection Kit at Laboratory Scale M Nurjayadi1., S F Jinan1., T Setiyoto1., D Hardianto2., A Sulfianti2., K Agustini2., D Sukmawati3., HA El-Enshasy4 1 Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Negeri Jakarta Gedung KH. Hasjim Asj’ari, 6th Floor, Jl. Rawamangun Muka Jakarta Timur, 13220, Indonesia 2 Biofarmaka Research Laboratory LABTIAP BPPT-Serpong, Tangerang Selatan, Banten, Indonesia 3Department of Biology, Faculty of Mathematics and Natural Science, Universitas Negeri Jakarta Gedung KH. Hasjim Asj’ari, 9th Floor, Jl. Rawamangun Muka Jakarta Timur, 13220, Indonesia 4Institute of Bioproduct Development, Universiti Teknologi Malaysia (UTM), Skudai, Johor Bahru, Malaysia Abstract Salmonella typhi is gram-negative bacteria that caused typhoid fever in humans; the disease prevention is currently carried out by vaccination. In addition to vaccination, the development of disease detection tools is also being carried out so that the detection process is faster and more accurate. In line with the development of typhoid detection devices, previous studies have managed to find factors that influence the production of Fim-C S. typhi protein on a small scale as raw material for typhoid detection kits. The purpose of this research is to apply the results of previous studies in the production of recombinant Fim-C S. typhi proteins with volume variations of 50mL-300mL, as a foundation for large-scale production. The results of protein production were characterized by SDS-PAGE and protein concentration measurements using the BCA Assay at a wavelength of 562 nm. The results showed an amount of that protein increase along with gradually production volumes, mainly the protein in the form of inclusion bodies. Pursuant to the results obtained can be concluded production of the recombinant protein Fim-C-S. typhi at 50-300 mL volume variation on a laboratory scale has been successfully performed with consistent results, which is expected to be basic in production at pilot scale and large scale. Keywords: Salmonella typhi, typhoid disease, Detection Kit, Fimbriae-C Recombinant Protein, Protein Production, Laboratory Scale. Share Link | Plain Format | Corresponding Author (Muktiningsih Nurjayadi)

56	Chemistry	ABS-627
The Relationship of Self-Regulated Learning with Student Learning Outcomes on Acid Base Topic: An Exploration of Flipped Classroom Learning Model Maria Paristiowati (a*), Arif Rahman (a), Sukro Muhab (a), Fatasya Kamal (a), Rinaldi Idroes (b) a) Department of Chemistry Education, Universitas Negeri Jakarta Jl. Rawamangun Muka, Jakarta 13220, Indonesia *maria.paristiowati[at]unj.ac.id b) Department of Chemistry, Universitas Syiah Kuala Kopelma Darussalam, Banda Aceh 23111, Indonesia Abstract Self-regulated learning plays an important role in students’ learning to organize their own learning activities to improve academic achievement. This study aimed is to analyze the relationship between self-regulated learning and students’ academic achievement through flipped classroom in acid-base learning. The study employed quantitative methods of correlational associations. The research sample of 51 students was selected by simple random sampling technique. The analysis prerequisite test shows normally distributed data with non-homogeneous variants. Hypothesis testing was employed by the non-parametric Spearman Rank correlation analysis. Correlation coefficient values obtained r = 0.416, which indicates that the moderate level of the correlation coefficient. Therefore, there is a positive relationship between self-regulated learning and students’ learning outcomes through a flipped classroom in acid-base learning. The determinant coefficient value is 17.3%, which shows that 17.3% of student learning outcomes are determined by self-regulated learning. This study also found that there was an increase in students self-regulated learning scores before and after learning with the flipped classroom. Based on a paired sample t test, the value of t count <t table was obtained. As a result, there is a correlation between self-regulated learning of students’ achievement in acid base learning with flipped classroom. Therefore, the flipped classroom can improve students self-regulated learning abilities. Keywords: Self-regulated learning, student learning outcomes, flipped classroom Share Link | Plain Format | Corresponding Author (Maria Paristiowati)

57	Chemistry	ABS-883
Physical quality of some type of grass on mixed planting with legum arachis and organic fertilizing Bye Feri Umbu Kaledi Wawu, I Dewa Nyoman Sudita *, I Gusti Ayu Dewi Sri Rejeki Faculty of Agriculture, Warmadewa University, Denpasar, Bali, Indonesia * E-mail: idnsudita[at]gmail.com Abstract The study used a Randomized Block Design with a factorial pattern consisting of 3 types of grass planted with Arachis (factor A) and 2 types of solid and liquid organic fertilizer (factor B) with 3 replications. The treatment combinations are R1C (Paspalum notatum grass with Arachis pintoi+Compost), R1B (Paspalum notatum grass with Arachis pintoi+Biourine), R2C (Paspalum conyugatum grass with Arachis pintoi+Compost), R2B (Paspalum notatum grass with Arachis pintoi+Biourine), R3C (Paspalum conyugatum grass with Arachis pintoi+Compost), R3B (Paspalum conyugatum grass with Arachis pintoi+Biourine). The results showed no significant difference both in planting a mixture of grass with legume and applying organic fertilizer to the physical quality of forage grass. In planting Axonopus compresus grass mixture shows the best average dry matter, water regains capacity (WRC), and water solubility (WS). Whereas the compost fertilizer gets a higher average value than biourine for dry matter, WRC, and WS. When looking at the combination of mixed planting with fertilization, compost fertilizer showed the highest average especially water solubility (WS). From this study, it can be concluded planting a mixture of grass and legume given compost fertilizer averaged better than the physical quality of the grass but not significantly different from all treatments. Keywords: mixed planting, organic fertilizer, physical quality Share Link | Plain Format | Corresponding Author (I Dewa Nyoman Sudita)

58	Chemistry	ABS-884
THE EFFECT OF BREWING TIME FOR pH VALUES, POLYPHENOLS CONTENT, and ANTIOXIDANT ACTIVITIES OF COFFEE’S PEEL TEA (CASCARA TEA) Shinta Maharani (a)*, Ika Mustikawati (a), Lili Nailufhar (a), Sarah Istiqomah Widiaputri (a) a) Program Study of Agroindustrial Technology Education, Universitas Pendidikan Indonesia Abstract Coffee’s peel tea or well known as Cascara tea is a by-product of coffee processing. It contains phenol compounds that has antioxidant activity. Total phenol compound is extracted by brewing process. It called polyphenol compounds commonly. Polyphenols extracted process deeply connected to brewing method, especially the time. The purpose of this study was to determine the effect of brewing time on pH, polyphenol content, and antioxidants activity of cascara tea. The method used in this study is a Completely Randomized Design with a variation of brewing time consisting of 5 levels, namely 5 minutes, 7 minutes, 10 minutes, 12 minutes and 15 minutes. The results showed that the variation of brewing time significantly affected the levels of polyphenols content, antioxidant activity, and pH values ​​of Cascara tea. The brewing time for 10 minutes has the highest levels of polyphenols content and antioxidant activity. Meanwhile pH values was decreased during brewing process Keywords: antioxidant activity, breweing time, cascara tea, polyphenols content Share Link | Plain Format | Corresponding Author (Shinta Maharani)

59	Chemistry	ABS-375
Utilization of Water Hyacinth Assisted Biofilter to Reduce Ammonia Levels In Tofu Liquid Waste Dewi Yunia, Yulia Sukmawardani, Cucu ZS Universitas Islam Negeri (UIN) Sunan Gunung Djati Bandung Abstract Phytoremediation is used by using aquatic plants in reducing the levels of pollutants from a waste. The type of plant used is adjusted to the pollutants that will be lowered. In addition, biofilter can also reduce levels of pollutants. This study aims to reduce the ammonia levels in liquid waste that are known by utilizing water hyacinth plants as phytoremediation and the use of wasp nest biofilter for the use of grade substitutes. Tofu wastewater treatment using biofilter and phytoremediation process is carried out with a variety of residence times. Ammonia content analysis in this study used the kjeldahl method consisting of three layers such as destruction, distillation, and titration. The type of titration used in this method is acid-base titration. The result of waste treatment using phytoremediation assisted by biofilter and analyzed using the kjeldahl method showed that variations in residence time for 8 days were more effective in reducting ammonia levels to reach a percentage above 87%. This show that the use of water hyacinth and biofilter is effective in reducing ammonium levels in tofu liquid waste. Keywords: amonia, tofu liquid waste, water hyacinth, wasp nest biofilter, kjeldahl method. Share Link | Plain Format | Corresponding Author (Dewi Yunia)

60	Chemistry	ABS-892
Antioxidant Activity Evaluation of Monofloral Honeys using Electroanalytical Approach B. Asih Suliasih1, Rizqi Aulia Tawwabin2, Setia Budi2,* 1Faculty of Pharmacy, Universitas Indonesia, Depok, INDONESIA 2Department of Physics, Faculty of Mathematics and Science, Universitas Negeri Jakarta, Jl. Rawamangun Muka, Jakarta 13220, INDONESIA Abstract The objective of this study was to develop antioxidant compound analysis technique by using electroanalysis approach. Five different samples from five monofloral honey were used in this work. The electroanalysis experiments were conducted using two different techniques, namely cyclic voltammetry (CV) performed with scan rate of 50 mV/s and different pulse voltammetry (DPV) with pulse amplitude 50 mV and scan rate 10 mV/s. All of the samples were also analysed by spectroscopy techniques, namely Ferric Reducing Ability of Plasma (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH). The electrochemical index determined using DPV uyiiuiouiitechnique agreed with the spectrometric FRAP and DPPH measurements. This result shows that the electroanalysis method could be potentially utilized for antioxidant analysis of honey samples. Keywords: Electroanalytical Technique, Antioxidant Activity Evaluation, Monofloral Honey Share Link | Plain Format | Corresponding Author (Setia Budi)

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