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61	Chemistry	ABS-894
COMPOSITION OF SOME FLAVONOID COMPOUNDS OF Selaginella SPECIES IN MOUNT MERAPI NATIONAL PARK AREA Sri Rahayu, Isfi Zahara, Rahmah Aulia Azzahrah, Hafidzah Zahratunnisa, Farid Pujiono Department of Biology, Faculty of Mathematics and Natural Science, Universitas Negeri Jakarta, Indonesia Abstract Selaginella is one of the genera of terrestrial plants that grow in humid area and can be used as a medicinal plant because it contains flavonoid such as biflavonoid. This substance are antioxidants and can prevent cancer. Mount Merapi National Park is an area protected by terrestrial ecosystems with an environment that supports the growth of fern plants such as Selaginella. This study aims to determine the levels of flavonoids of Selaginella species in the Kali Kuning and Kaliurang area of Mount Merapi National Park. Seven sample were obtained from field observation. The seven samples identified as S. opaca, S. plana, S. involvens, SP 1, and SP 2 in Kaliurang region and S. involvens and S.eurynota iin Kalikuning region. Highest water content were found in S. opaca (62%) and the lowest water content on S. plana (27%). Samples are extracted using acetone by participating 1: 6 for 48 hours. The highest yield found in S. eurynota (57.89%) while the lowest yield found in Selaginella SP 1 (12%). Qualitative detection of flavonoid content was done using TLC test. The eluent used consisted of ethanol:chloroform (1: 9) and showed visible yellow result. All Selaginella samples positive contain flavonoids. Measurement of flavonoid levels using ascorbic acid as a standard with the formula 2, 4, 6 and 8 ppm. The largest flavonoid content obtained in S. plana (69.283%) while the lowest level was obtained in S. eurynota (18.371%). Keywords: flavonoid content, water content, extraction, TLC test Share Link | Plain Format | Corresponding Author (Isfi Zahara)

62	Chemistry	ABS-899
Honey, candy, and water: which one is the most effective in neutralizing spicy sensation? Ambar Sulianti, Rizqiatul Badriah, Muhammad Naufal Fadlurrahman Riyadhi UIN Sunan Gunung Djati Abstract Many Indonesian people are fond of the spicy flavor caused by capsaicinoids in chili. However, once they are spicy, they experience interference in focus so it needed to be quickly neutralized. This study aims to analyze which of the honey, candy, and water are most effective for neutralizing spicy sensations. This study used an experimental method to 100 people who were given chips mixed with chili. Test materials in this study were pure livestock honey, coffee candy, and water. We used stop watch to measure the time needed to neutralize the sensation of spicy. Since the data were normally distributed, we used statistical repeated ANOVA tests. The results showed that honey neutralized the spicy taste in 26.6 seconds, candy 43.03 seconds and water 40.6 seconds. There is a significant difference in the speed of loss of the spicy taste in mineral water, honey, and chocolate candy. This research shows among the test materials in this study, honey is the fastest in neutralizing the spicy taste. Keywords: Honey, candy, water, spicy, sensation Share Link | Plain Format | Corresponding Author (Ambar Sulianti)

63	Chemistry	ABS-133
The Study of Structural Properties and Photocatalytic Activity of ZnO Prepared by Ultrasonic Assisted Precipitation Method Sanusi[1], Soni Setiadji[1,*], Nanda Dwimar Hakim[1], Syuqron Habib Ramadhana Al Aziz[1], Asti Sawitri[2], Hasniah Aliah[2], Eko Prabowo Hadisantoso[1], Atthar Luqman Ivansyah[3] and Dani Gustaman Syarif[4] [1]Department of Chemistry, Faculty of Science and Technology, UIN Sunan Gunung Djati Bandung, Jl. A.H. Nasution No.105 Bandung, West Java, 40614, Indonesia. [2]Department of Physics, Faculty of Science and Technology, UIN Sunan Gunung Djati Bandung, Jl. A.H. Nasution No.105 Bandung, West Java, 40614, Indonesia. [3]Master Program in Computational Science, Faculty of Mathematic and Natural Science, InstitutTeknologi Bandung, Jl.Ganesha No. 10, Bandung, West Java, 40132, Indonesia [4]Lab. of Material Physics, Badan Tenaga Atom Nasional, Jl. Taman Sari Bandung No. 71, West Java, 40132, Indonesia. Abstract ZnO is a semiconductor that has excellent activity as photocatalyst under UV light region. ZnO is precipitated by adding oxalate acid onto zinc acetate dehydrate solution with ultrasonic assisted at various times and flattened at 500 °C. The structural properties of the ZnO samples are characterized by X-Ray Diffraction (XRD) and Brunauer-Emmett-Teller (BET). XRD result has confirmed the ZnO structure as hexagonal phase. Rietvield refinement of XRD pattern by La Beil method is employed to analyze the lattice parameters of ZnO. It provides that ZnO as wurtzite with accurate values. Debye-scherrer equation examines the crystallite size. It is confirmed that the crystallite size of ZnO is decreased by ultrasonic assisted at precipitation. BET result shows that ultrasonic assisted could increase the surface area of ZnO, which are 45.64 m2/g of ZnO without ultrasonic assisted and 143,487 m2/g of ZnO with ultrasonic assisted along 2 h. The photocatalyst experiment is set up to analyze the photocatalytic activity of ZnO for degradation of methylene blue under UV light irradiation. By significantly that decolorization of methylene blue occurred at pH 10 for 30 minutes irradiation. The photocatalytic activity for ZnO samples with 1 h and 2 h ultrasonic assisted are 79.54 % and 86.50 % respectively, whereas ZnO sample without ultrasonic assisted is 70.90 %, and almost all of methylene blue contaminant have done degraded successfully after 120 min irradiation. According to the results, the structural properties and photocatalytic activity of ZnO are increased as consequence to the presence of ultrasonic assisted on precipitation. Keywords: ZnO nanoparticles, ultrasonic-assisted, precipitation, photocatalyst, rietvield-refinement Share Link | Plain Format | Corresponding Author (Soni Setiadji)

64	Chemistry	ABS-390
Analysis of Cyclamate Sweeteners in Elementary School Students Drink in South Denpasar Subdistrict Ni Made Ayu Suardani Singapurwa, I Dewa Ayu Ketut Yunantariningsih, I Wayan Sudiarta, I Putu Candra, A.A. Made Semariyani Department of Food Science and Technology, Warmadewa University Abstract Cyclamate is an artificial sweetener with a sweetness level of 30-40 times greater than sucrose. This study aimed to determine the content and levels of cyclamate sweetener in unmarked beverages without labels that are prepared and sold by elementary school canteens in South Denpasar District. This research is a type of descriptive research that uses survey methods with simple random sampling techniques and experiments in the laboratory. The number of elementary schools in South Denpasar District is 72 schools. The population in this study were 145 types of unmarked beverages without labels that were prepared and sold by elementary school canteens in South Denpasar District. The number of samples sampled is 110 samples. The parameters of artificial sweeteners in this study were cyclamate tested by HPLC. Research data are presented in tables, graphs and analyzed descriptively. The results showed that of 110 samples containing 62 cyclamate (56.36%). Cyclamate levels that exceed the maximum limit requirements so that they do not meet the requirements of 44 samples (40%) and meet the requirements of 66 samples (60%), according to the maximum allowable limits refer to the Head of the Indonesian National Agency for Drug and Food Control No. 4 of 2014. Keywords: artificial sweeteners, cyclamate, hawker drinks Share Link | Plain Format | Corresponding Author (Ni Made Ayu Suardani Singapurwa)

65	Chemistry	ABS-135
Analysis of Taste in Tofu from Lembang, Sumedang and Garut West Java Assyifa Junitasari (a*), Fida M.Warganegara (b), Made Puspasari Widhiastuty(C) a) Chemistry Departement, Faculty of Sains and Technology, UIN Sunan Gunung Djati Bandung, Jl. A.H. Nasution No. 105, Cipadung, Bandung 40614, Indonesia *assyifajunitasari[at]uinsgd.ac.id b) Chemistry Departement, FMIPA, ITB, Jl. Tamansari No.64, Bandung 40116, Indonesia Abstract Tofu from Lembang, Tofu from Sumedang and Tofu from Garut are three types tofu that are produced in different places, but still close. Although made from the same raw material that is soy, every tofu will produce a different tofu, both in terms of taste, aroma, texture and suppleness. Differences in the type of flavour compouds used can also be a factor causing divergence of any tofu product. The characteristic of the tofu were determined by identifying the flavour compouds GC-MS, as well as organoleptic tests. To determine differences in compound-producing falvour in the tofu. The results extraction method was carried out on tofu samples using n-pentane as solvent then analyzed using GC-MS. Flavour compounds of all three types of tofu included into the ester group, alkenes and aldehydes. Organoleptic tests show that the panelists prefered. Tofu from Sumedang for test of colour appearance (82%), for the test texture panelists like Tofu from Garut (83%), for test of elasticity panelists prefered Tofu from Garut Lembang (75%), for taste test panelists prefered Tofu from Lembang (88%), panelists prefered Tofu from Lembang for flavour test (82%). Keywords: Tofu;GC-MS;Organoleptic tests Share Link | Plain Format | Corresponding Author (Assyifa Junitasari)

66	Chemistry	ABS-903
ISOLATION FLAVONOID COMPOUND CHRYSOERIOL FROM ETHYL ACETATE EXTRACT OF ZAITUN LEAVES (Olea europaea) Asep Supriadin (a*), Hera Juliani (a), Nila Tanyela(b) a)Faculty of Science and Technology, UIN Sunan Gunung Djati Bandung *asupriadin[at]uinsgd.ac.id b) Chemistry Department, Pertamina University Abstract ISOLATION FLAVONOID COMPOUND CHRYSOERIOL FROM ETHYL ACETATE EXTRACT OF ZAITUN LEAVES (Olea europaea) The Olive Tree (Olea europaea) is a plant originating in the Middle East. Olea Europaea plant is contained several secondary metabolites such as olive oil, phenolic compounds, flavonoids, triterpen, biphenol. The aims of this research are to isolate and identify a flavonoid compound that extracted in the ethyl acetate of olive leaves. Isolation was carried out by meseration, liquid vacuum chromatography, column chromatography and thin layer chromatography. Isolation results obtained 10 mg isolates were identified using proton nuclear magnetic spectroscopy (1H-NMR) and carbon (13C-NMR). According to spectra analysis, describe that isolate is chrysoeriol. Keywords: Olea europaea; flavonoid; chrysoeriol Share Link | Plain Format | Corresponding Author (Asep Supriadin)

67	Chemistry	ABS-136
Biodiversity of Prokaryotic Microorganisms in Whey Tofu from Lembang, Sumedang and Garut West Java Assyifa Junitasari (a*), Fida M.Warganegara (b), Made Puspasari Widhiastuty(C) a)Chemisrty Departement, Faculty of Sains and Technology, UIN Sunan Gunung Djati Bandung, Jl. A.H. Nasution No. 105, Cipadung, Bandung 40614, Indonesia *assyifajunitasari[at]uinsgd.ac.id b)Chemistry Departement, FMIPA, ITB, Jl. Tamansari No.64, Bandung 40116, Indonesia Abstract Tofu is one of the products processed from soybeans that contain nutrients for the body. Tofu from Lembang, Tofu from Sumedang and Tofu from Garut are three types tofu that are produced in different places. Every tofu will produce a different tofu, both in terms of taste, aroma, texture and suppleness. This difference could be due to differences in treatment during the manufacturing of tofu. Differences in the type of coagulant used can also be a factor causing divergence of any tofu product. Whey, which is known as a by product of tofu, it can be function as a coagulant in the tofu making process. Types of microorganisms found in whey can influence the flavor of tofu. This research was conducted to find out the type of microorganisms contained in whey Tofu from Lembang, Sumedang and Garut through metagenom approach. Through this approach, the microbial species in an environment can be known, even without the need to cultivate the microorganism. Whey samples filtered by cellulose acetate membrane of 0.2 µm. Total DNA was isolated by the Klijn method, and used as a template to obtain a fragment of 16S rRNA with ± 400 bp long using touchdown PCR. Amplicons obtained then separated again by DGGE analysis. Optimization of DGGE was made with 8% gel and a denaturant range of 30%-80%. Bacterial diversity information was obtained by comparing the DNA sequences of 16S rRNA gene samples with GenBank database and phylogenetic analysis. The results of the homology analysis based on nucleotides sequence on each band showed that the microorganisms contained in Tofu from Lembang, Sumedang and Garut were different. The microorganisms contained in whey Tofu from Lembang have closer homology to the Bacterium EM, microorganisms contained in Tofu from Sumedang were closer with Acinobacter sp. and Uncultured Bacterium, while microorganisms contained in Tofu from Garut were closer to Uncultured bacterium. Keywords: Biodiversity; Microorganisms; Whey, 16S rRNA; DGGE Share Link | Plain Format | Corresponding Author (Assyifa Junitasari)

68	Chemistry	ABS-912
Molecular Docking Study of Inclusion Complex between Aromatic Amine and Calixarene Analogs Atthar Luqman Ivansyah1,2), Citra Deliana Dewi Sundari3), Soni Setiadji4), Tety Sudiarti4) 1) Master Program in Computational Science, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Jl. Ganesha No. 10, Bandung, West Java, 40132, Indonesia 2) Analytical Chemistry Research Group, Department of Chemistry, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Jl. Ganesha No. 10, Bandung, West Java, 40132, Indonesia 3) Department of Chemistry Education, UIN Sunan Gunung Djati Bandung, Jl. Cimincrang, Cimenerang, Panyileukan, Bandung, West Java, 40292, Indonesia 4) Department of Chemistry, UIN Sunan Gunung Djati Bandung, Jl. A.H. Nasution No.105, Bandung, West Java, 40164, Indonesia Abstract A molecular docking study done in this research by using ArgusLab version 4.0.1 software package was used to explore the capability of Calixarene Analogs for Aromatic Amine extraction. The Calixarene Analogs used in this research are Calixpyrrole, Calixpyridine, Thiacalixarene, Heterocalixaromatic, Calixcarbazole and Calixnaphtalene; and the Aromatic Amine used in this research are Aniline, 4-Chloroaniline, Toluene-2,4-diamine, 2-Naphtylamine, 4,4’-Metylenbis(2 chloroaniline), 4,4’-Metylendianiline, N-Nitrosodiphenylamine, Benzidine, 2-Aminobiphenyl, 2 Amino-1-methyl-6-phenylimidazo[4,5-b] Pyridine, 3-Trifluoromethylaniline, p-Phenylendiamine, o-Toluidin, 4-Chloro-o-toluidin. In this molecular docking simulation, the guest molecule of Aromatic Amine was treated as ligand and host molecule of Calixarene Analogs was treated as binding site. This molecular docking simulation used AScore scoring function, binding sitebox size for host molecule of 20Å x 20Å x 20Å, with grid resolution of 0.4Å, docking engine of ArgusDock with high precision, no augmented torsions, flexible ligand with maximum number of poses of 200. Keywords: Molecular docking, ArgusLab, Calixarene, Aromatic Amine Share Link | Plain Format | Corresponding Author (Atthar Luqman Ivansyah)

69	Chemistry	ABS-401
Investigating the potency of bioactive compounds from Ficus religiosa as anti-inflammatory agent Wiji Utami (a*), Hafiz Aji Aziz (b), Dindin Nasrudin (c) a) Chemistry Department, Faculty of Science and Technology, UIN Sulthan Thaha Saifuddin, Jambi, Indonesia. *wijiutami[at]uinjambi.ac.id b)Austrian-Indonesian Centre (AIC) for Computational Chemistry, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, Indonesia c)Department of Physics Education, UIN Sunan Gunung Djati, Bandung, Indonesia. Abstract The previous studies explain that bioactive compounds from Ficus religiosa are known as an anti-inflammatory agent. In this research, we investigated the potency of bioactive compounds from Ficus religiosa by using molecular docking simulation between 10 bioactive compounds and the COX-2 receptor. The ten ligands were collected from www.pubchem.ncbi.nlm.nih.gov., while the receptor was taken from www.rcsb.org. The result of this study could be a reference for the research in the synthesis of the bioactive compound to minimize failure. The collected data was calculated through Autodock Vina embedded in MGL Tools 1.5.6, and these processes were performed using 100 runs of Lamarckian Genetic Algorithm (LGA). The lowest energy of complexes was visualized by using Biovia Discovery Studio Visualizer. This result proved that β-amyrin had smaller binding energy compared to the native ligand, and tied to TYR385 nad SER530 residues. Keywords: anti-inflammatory, ficus religiosa, molecular docking Share Link | Plain Format | Corresponding Author (Wiji Utami)

70	Chemistry	ABS-659
Carcass Fractures of 10-week Old Native Chickens Given Ration Containing Fermented Cassava Flour Luh Suariani *, Ni Ketut Etty Suwitari, Ni Made Yudiastari, Nyoman Kaca, Yan Tonga Department of Animal Husbandry, Faculty of Agriculture, Warmadewa University, Jl. Terompong 24, Denpasar, Bali. 80235. Indonesia. Abstract One of the local feeds that can be utilized as native chicken feed is Gaplek. Gaplek is made from cassava, but the nutritional content is still low. For this reason, fermentation needs to be done so that the nutritional value increases. The purpose of this study was to look at the effect of giving rations containing fermented cassava flour to 10-week old chicken carcass fractures. The design used in this study was a Completely Randomized Design with five treatments (P0 = ration without fermented cassava flour (TGF), (P1 = ration with 10% TGF, P2 = ration with 20% TGF, P3 = ration with 30% TGF and P4 = ration with 40% TGF) and three replications. Each replication consisted of 5 chickens. The observed variables were breast weight, thigh weight, wing weight, and back weight. The results showed that the provision of fermented cassava flour to a level of 20% was significantly able to increase breast weight, back weight and wing weight of 10-week-old native chicken, but it gives results. not significant on back weight, it is recommended to use fermented cassava flour to 20% in the ration to get the best results. Keywords: Carcass Fractures, Native chicken, Fermented Cassava Floor, Fermentation Share Link | Plain Format | Corresponding Author (Luh Suariani)

71	Chemistry	ABS-665
Phytochemical and Antioxidant Capacity Test on Turmeric Extract (Curcuma longa) Traditionally Processed in Bali P A N K Permatananda1, A A S A Aryastuti1, P N Cahyawati1, D P C Udiyani1, D Wijaya1, I G S Pandit2,3, A A N M Wirajaya2 1Faculty of Medicine and Health Science Universitas Warmadewa 2Faculty of Agriculture Universitas Warmadewa 3Postgradute Program Universitas Warmadewa Abstract Bali is an islant that is famous for its culture, including traditional medicine. In traditional medicine, the Balinese use various kinds of medicinal plants, one of which is Turmeric. The purpose of this study was to determine the ways and objectives of the use of turmeric by Balinese, as well as the phytochemical content and antioxidant capacity of turmeric extract which is traditionally processed in Bali. The method and purpose of utilizing turmeric were obtained through observation and interviews with 900 Balinese respondents. Quantitative phytochemical tests include starch, protein, flavonoid, tannin, phenol and vitamin C levels and qualitatively for the presence of triterpenes, steroids, alkaloids, and saponins. Antioxidant capacity was measured using the DPPH method. Through this research, we found there were only 36.8% of respondents had ever used turmeric as a traditional medicine. Utilization of turmeric was mostly in the form of loloh or traditional drinks. Phytochemical test results showed turmeric extract had 67.38% starch, 3.42% protein, 2709.39 mg / 100 gr flavonoids, tannins 291.64 mg / 100gr, phenol 1584.04 mg / 100 gr, and vitamin C 0.06 mg / 100gr. Qualitatively, turmeric extract contained triterpenes, alkaloids, and saponins, but did not contain steroids. The antioxidant capacity of turmeric extract was 70.9 mg / L GAEAC. Turmeric extract is a traditional medicine made from nature that is most commonly used by Balinese and very potential to be developed as an antibacterial, antioxidant, anti-inflammatory, or other benefits that still need further investigation. Keywords: Phytochemical, Antioxidant Capacity, Turmeric, Bali Share Link | Plain Format | Corresponding Author (Pande Ayu Naya Kasih Permatananda)

72	Chemistry	ABS-922
The Comparison Characteristics of Fermented Virgin Coconut Oil (VCO) on Kefir Addition M Karlina, N Windayani*, I Helsy Chemistry Education Department, UIN SUnan Gunung Djati Bandung. Jl. AH Nasution No. 105 Bandung 40614, Indonesia Abstract Indonesia is the largest coconut producing country in the world, one of the processed coconut products which includes functional food, has many benefits in the pharmaceutical and beauty field, can be made with easy and inexpensive procedures, also has high economic value is Virgin Coconut Oil (VCO). So far, no literature has been found in making VCO with kefir starter, so the purpose of this study is to describe the comparison of the characteristics of VCO produced from the fermentation process with the addition of kefir based on organoleptic tests, water content, and free fatty acid levels. Experimental research method with two replications design. Three sample coconut milk canil fermented 48 hours with the addition of different starters ie 0% (control), 3.5% and 6% kefir. The experimental results obtained by VCO as much as 50-70 mL (11-16% of the amount of thick coconut milk per sample) with colorless characteristics, normal taste, and aroma of coconut aroma, with a moisture content of 0.0028% and a free fatty acid content of 0.23%. The VCO produced is in accordance with SNI VCO 7381: 2008. Further research will be carried out optimization to produce an optimal VCO. Keywords: free fatty acids, fermentation, water content, kefir, organoleptic, VCO Share Link | Plain Format | Corresponding Author (Neneng Windayani)

73	Chemistry	ABS-924
Antioxidant Activity of Litsea petiolata Hk. f Neneng Siti Silfi Ambarwati(a), Berna Elya(b), Putu Gita Maya Widyaswari Mahayasih(c), Muhamad Sharul Nizam Awang(d), Hanita Omar(d)* a) Department of Cosmetology, Faculty of Engineering, Universitas Negeri Jakarta, Jl. Rawamangun Muka, Jakarta Timur, DKI Jakarta, 13200, Indonesia. b) Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, West Java, Indonesia. c) Department of Pharmacy, Faculty of Health and Sciences, Universitas Esa Unggul, Jakarta, Indonesia. d) Chemistry Division, Centre for Foundation Studies in Science, University of Malaya, Kuala Lumpur, 50603, Malaysia Abstract Background. Litsea petiolata Hk. f was included Lauraceae family, and the previous study had been isolated 5 compounds from the Litsea petiolata Hk. f stem bark dichloromethane extract namely harman or aribine, norharman, reticuline, isoboldine, and thalifoline. Antioxidants can prevent tissue damage by free radical. Free radical production continuously in all cells as cellular function usually, but excess production can cause many diseases. Objective. The research aimed to assay the activity of antioxidant from the extract and fractions of the Litsea petiolata Hk. f stem bark with DPPH assay and FRAP assay. Methods. The extract was obtained by soxhletation and used dichloromethane as the solvent. The fractions were fractionated with column chromatography. The antioxidant test used DPPH assay and FRAP assay. Results. The IC50 values for the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging test of the dichloromethane extract was 27.36 µg/mL, the fraction A was 113.74 µg/mL, fraction B was 60.17 µg/mL, and the control positive (quercetin) was 3.96 µg/ml. The EC50 values for ferric ion reducing antioxidant potential (FRAP) test of the dichloromethane extract was obtained 13.47 µg/mL, the fraction A was 76.49 µg/mL, fraction B was 55.73 µg/mL, and the control positive (quercetin) was 14.01 µg/ml. Conclusion. The extract had higher antioxidant activity than the fractions, and by FRAP test the extract showed better antioxidant activity than the positive control (quercetin). Keywords: Litsea petiolata Hk. f, stem bark, maceration, antioxidant, DPPH, FRAP Share Link | Plain Format | Corresponding Author (Neneng Siti Silfi Ambarwati)

74	Chemistry	ABS-669
Development of Media Mobile Learning Based on Scientific Approach to Solubility and Solubility Product Ucu Cahyana(a*), Rahma Dwi Pasha(a), Rifkah Nabilah(a), Arif Rahman(a) a) Chemistry Education Faculty of Mathematics and Natural Sciences Universitas Negeri Jakarta *) ucahyana[at]unj.ac.id Abstract The scientific approach is mandatory for the 2013 curriculum. Mobile learning makes students be more active. This study aimed to develop mobile learning on solubility and solubility product material applying the scientific approach and to know the feasibility of developed media. This study was conducted using the method of Research and Development Borg & Gall in SMA 4 Bekasi and SMA PGRI 1 Bekasi in the 2018/2019 academic year. Results of the feasibility percentage-based due diligence by media expert at 93,4% with 0,83 of reliability. Result of judicial review and the language of 80,3% with 0,64 of reliability. Result of small-scale trials by students of 85% and by teachers of 97%. Result of large-scale trials by students of 86% and of 97,8% by teachers. The conclusion from results testing experts and testing by students and teachers is mobile learning already meets the criteria and fit for use by the needs of students and teachers Keywords: Mobile Learning, Scientific Approach, Solubility and Solubility Product Share Link | Plain Format | Corresponding Author (Ucu Cahyana)

75	Chemistry	ABS-670
Development of Android-based Mobile Media Learning Through a Scientific Approach on Electrolyte Solution and Redox Reactions. Ucu Cahyana(a*), Windha Utari(a), Ella Fitriani(a) a) Chemistry Education Faculty of Mathematics and Natural Sciences Universitas Negeri Jakarta *) ucahyana[at]unj.ac.id Abstract Development in science and technology demanding education to participate for using technology as a form of innovation in learning. This study aims to develop innovative learning media that is mobile learning through scientific approaches on Electrolyte and Redox topic and to know the feasibility of media produced. This research was conducted at SMAN 39 Jakarta and SMAN 98 Jakarta from January to May 2019. The research method used is Research and Development (Borg and Gall) by modifying five stages, namely: needs analysis, product development, validation, and product test. The resulting mobile learning media is called "Electrolyte and Redox.apk” compatible on Android devices which provide a summary, videos, simulations, quizzes, and educational games. Feasibility test by topic and language expert acquired 83%-100% with 0.808 of reliability. Feasibility test by media experts acquired 91%-100% with 0.812 of reliability. Media trials by chemistry teachers acquired 88% -100% with “very good” criteria. Media trials by small scale students acquired 88% and large scale acquired 92% with "very good" criteria. Based on the results can be concluded that mobile learning media through the scientific approach in Electrolyte and Redox topic proper to use as a learning media and suitable for students and teachers needed. Keywords: Mobile learning, Scientific Approach, Electrolytic and Redox Share Link | Plain Format | Corresponding Author (Ucu Cahyana)

76	Chemistry	ABS-159
TOXICITY OF ESSENTIAL OILS FROM ORANGE (Citrus sinesis L. obbeck) AND LEMONGRASS (Cymbopogon nardus L. Rendle) ON Aedes aegypti A VECTOR OF DENGUE HEMORRHAGIC FEVER (DHF) Nunung Kurniasih1*, Wildan Nuryadin1, Mardi Nazrin Harahap1, Asep Supriadin1, Ida Kinasih2 1Department of Chemistry, Faculty of Sciences and Technology, Sunan Gunung Djati Islamic State University, Bandung 40614, Indonesia 2Department of Biology, Faculty of Sciences and Technology, Sunan Gunung Djati Islamic State University, Bandung 40614, Indonesia Abstract Aedes aegypti mosquito is one of common vector for the pathogens of different diseases like dengue hemmorrhagic fever. Most common approach to vector-borne disease is by chemical control, mostly through the use of insecticides. As compared to synthetic pesticides, essential oils (EOs) are ecologically safe, have no mammalian toxicity or the chances of development of resistance are reasonable and highly popular with the organic growers. Essential oil from orange (Citrus sinesis obbeck l) and lemongrass (Cymbopogon nardus L. Rendle) known to contain citronellal, geraniol and citronellol. Citronellal compound acts as an insecticide ingredient that works as an antifeedant and repellent. Orange and lemongrass cleaned and cut into small pieces, dried and then blended to obtain crude drug samples. Samples distilled with Stahl distillation method for 4 hours. Furthermore, terpenoids phytochemical test and test against larvae of Aedes aegypti larvicides to obtain the LC50 value. The yield of essential oil of orange (Citrus sinensis L Obbeck) obtained is 6.84%, while the essential oils of lemongrass (Cymbopogon nardus L. Rendle) was 0.96%. In the phytochemical test essential oils either of orange peel and lemongrass showed terpenoids. Essential oil from orange and lemongrass are toxic, with LC50 values obtained from lemongrass 97.72 ppm is smaller than the orange is 790.935 ppm. The essential oil of lemongrass more toxic than the essential oil of orange. Keywords: orange (Citrus sinesis L Obbeck), lemongrass (Cymbopogon nardus L. Rendle), Test larvicidal effects, larvae of Aedes aegypti, Lethal Concentration (LC50). Share Link | Plain Format | Corresponding Author (Nunung Kurniasih)

77	Chemistry	ABS-160
Extraction and Characterization of Bixin from Anatto Seeds with microwave Erdawati and Edith Alanas Universitas Negeri Jakarta Abstract This study aims to determine the effect of extraction time (5,10,15 minutes) and the ratio of the aquadest solvent: ethyl acetate (1: 6.5, 1: 7, 1: 8 v / v) to the yield of pigment . The optimum condition was found on the ratio of solvent of distilled water: ethyl acetate (1: 7 v / v) extraction time for 15 minutes with low microwave power resulted in maximum pigment yield of 0.469%. The isolate compounds were analyzed using IR spectroscopy, mass spectroscopy, and High Performance Liquid Chromatography (HPLC) showed that the isolates were bixin pigments having molecular weight of 394 g / mol with wave numbers for OH groups, C = O carboxylate groups, CH bonds, duplicate C = C, and CO ester bonds. As well as showing the retention time of standard and standard pigments at minutes to 12.849 and 12.843 Keywords: extraction, bixin, seed annatto, microwave Share Link | Plain Format | Corresponding Author (Erdawati Bayazid)

78	Chemistry	ABS-672
Development of Mobile Media Learning on Scientific Approach in Stoichiometry Ucu Cahyana(a*), Refani Izqi Luthfianisa(a), Tritiyatma Hadinugrahaningsih(a) a) Chemistry Education Faculty of Mathematics and Natural Sciences Universitas Negeri Jakarta *) ucahyana[at]unj.ac.id Abstract This study purpose id for designing, developing, and testing the feasibility of mobile learning based on scientific approach, as the result can help teachers and students of class X in stoichiometry topic. This study teasted at SMAN 113 Jakarta and SMAN 48 Jakarta in 2018 /2019 academic year. Research and Development method by Borg and Gall used by modifying stages of need analysis, development, validation, and product test. The result of need analysis indicated necessary media which provide a summary, exercise, lab practice video, animation video, and games of education as learning based on scientific support in stoichiometry topic. Amount of 74% of respondents appreciated in learning using mobile. At the development phase designed mobile learning media and storyboard along with software chosen to make mobile learning. Feasibility test for media acquired 88% with 0.77 of reliability. Feasibility test for topic ang language acquired 88% with 0,9 of reliability. Chemistry teachers test acquired 92% with the best criteria. Small-scale student test acquired 81% and big-scale student test acquired 83% with the best criteria. According to this study concluded that mobile learning media based on scientific in stoichiometry topic is proper to use and suitable for student and teacher needed. Keywords: Development research, mobile learning, stoichiometry, scientific approach Share Link | Plain Format | Corresponding Author (Ucu Cahyana)

79	Chemistry	ABS-929
Computational Modeling of Inclusion Complex between Aromatic Amine and Calixarene Analogs using Quantum Semiempiric Method Atthar Luqman Ivansyah1,2), Soni Setiadji3), Citra Deliana Dewi Sundari4), , Tety Sudiarti3) 1) Master Program in Computational Science, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Jl. Ganesha No. 10, Bandung, West Java, 40132, Indonesia 2) Analytical Chemistry Research Group, Department of Chemistry, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Jl. Ganesha No. 10, Bandung, West Java, 40132, Indonesia 3) Department of Chemistry, UIN Sunan Gunung Djati Bandung, Jl. A.H. Nasution No.105, Bandung, West Java, 40164, Indonesia 4) Department of Chemistry Education, UIN Sunan Gunung Djati Bandung, Jl. Cimincrang, Cimenerang, Panyileukan, Bandung, West Java, 40292, Indonesia Abstract Computational modelling done in this research by using quantum semiempiric method and Gaussian 09 Rev. D 01 software package was used to explore the capability of Calixarene Analogs for Aromatic Amine extraction. The Calixarene Analogs used in this research are Calixpyrrole, Calixpyridine, Thiacalixarene, Heterocalixaromatic, Calixcarbazole and Calixnaphtalene; and the Aromatic Amine used in this research are Aniline, 4-Chloroaniline, Toluene-2,4-diamine, 2-Naphtylamine, 4,4’-Metylenbis(2 chloroaniline), 4,4’-Metylendianiline, N-Nitrosodiphenylamine, Benzidine, 2-Aminobiphenyl, 2 Amino-1-methyl-6-phenylimidazo[4,5-b] Pyridine, 3-Trifluoromethylaniline, p-Phenylendiamine, o-Toluidin, 4-Chloro-o-toluidin. Quantum semiempiric method used in this research were PM3, PM6, and PM7. Keywords: Quantum Semiempiric, Gaussian 09 Rev. D01, Inclusion complex, Calixarene, Aromatic Amine Share Link | Plain Format | Corresponding Author (Atthar Luqman Ivansyah)

80	Chemistry	ABS-162
Performance Column Adsorption of Methylene Blue Using Composite Spent Coffe Ground-Copper Ferrites SCG/ CuFe2O4 1Zulhipri 2Nadiroh and 3Erdawati Universitas Negeri Jakarta Abstract Composite Spent Coffe Ground-Copper Ferrites (SCG/CuFe2O4) were synthesized by a prepitation method. Copper nitrate, ferri nitrate, SCG and sodium hydroxide mixed and stirred at 80oC for 5h, then calcined at 600oC for 2 h. The resulting product is characterized by SAA This study aims to determine capacity adsorption of SCG/CuFe2O4 to adsorb Methylen Blue (MB) with a column system.Approximately 0.5 of SCG/CuFe2O4 were packed into the column. Infusion tube filled with 250 mL MB 20 mg.L-1 was adjusted to pH 10, passed through a column at flow rate 0.8 mL/min and 1.2 mL/min. The results obtained show that the slower the flow rate, the adsorption capacity (q0) and Thomas constant (kTh) Pb2 + ions increase. The highest q0 and kTh values were obtained at a flow rate of 0.8 L / min at 80.133 mg / g, 0.0028 L / mg. Minutes.Desorption of CuFe2O4 with 0.1 M HCl solution show that with 3 times elution, all MB which are bound to the adsorbent can be released Keywords: SCG/CuFe2O4, adsorbent, column method, methylene Blue.Thomas model Share Link | Plain Format | Corresponding Author (Erdawati Bayazid)

81	Chemistry	ABS-674
Integrating Dilemma Stories and STEAM Projects in Petroleum Topic for Problem-Solving Skills Development N Veronica, Y Rahmawati, and Erdawati Universitas Negeri Jakarta, Jalan Rawamangun Muka, Jakarta 13220, Indonesia Abstract This research focuses on the development of problem-solving skills by integrating dilemma stories with Science, Technology, Engineering, Art, and Mathematics (STEAM). The problem-solving skills as a set of abilities that students need to develop in facing the future challenge, especially to solve problems in their daily lives. This approach uses a dilemma story to understand chemistry concepts, followed by a STEAM project based on alternative fuel to find a solution to the problem presented in the story. The study was conducted in a secondary school on the topic of petroleum in year 11. A qualitative methodology was employed to explore students’ learning experiences and understanding of the research context. Data was collected through interviews, observations, reflective journals, and chemistry problem-solving tests. The theme of problem-solving skills was explored through data analysis. The results show that integrating dilemma stories to STEAM projects can encourage students to developed problem-solving skills in understanding the problem, strategizing, solving the problem, and evaluating the outcomes. The researchers faced the challenge of integrating a suitable chemistry-based dilemma story with a STEAM project while empowering students and managing time resources. Keywords: Chemistry Learning, Dilemma Stories Approach; STEAM Project; Problem Solving Skills Share Link | Plain Format | Corresponding Author (Yuli Rahmawati)

82	Chemistry	ABS-675
Identifying Chemistry Self-Concept Through the Culturally Responsive Transformative Teaching Model S. Izzati, Y. Rahmawati, A. Ridwan, R.E. Andina Faculty Mathematics and Natural Sciences, State University of Jakarta Jakarta, Indonesia Abstract This study sought to analyze students’ self-concepts in organic chemistry learning through the Culturally Responsive Transformative Teaching model, which consists of five steps: 1) identification, 2) cultural understanding, 3) collaboration, 4) critical reflection, and 5) construction transformation. This qualitative study was compiled using data from interviews, classroom observations, reflective journals, and an assessment instrument known as the chemistry self-concept inventory, which consists five categories: chemistry self-concept, mathematical self-concept, academic self-concept, academic enjoyment self-concept, and creativity self-concept. The study was conducted in a private high school in Bogor, Indonesia, with 42 students in grade 12. The results of the chemistry self-concept inventory test showed a various self-concept. Students who have a good self-concept in learning chemistry can show better abilities in understanding the concepts. One of the learning models that can be used to help students is the Culturally Responsive Transformative Teaching (CRTT) model. Based on the research results obtained, the understanding of self-concepts and the integration of the CRTT model can help shape the positive attitudes possessed by students. Keywords: Chemistry Self-concept; Cultural Responsive Transformative Teaching, Organic Chemistry, Carbon Compounds Share Link | Plain Format | Corresponding Author (Yuli Rahmawati)

83	Chemistry	ABS-676
Effect of spacing on growth and yield of several black soybean varieties (Glycine max L Merril) Krisna Maulana, Made Sri Yuliartini *, Anak Agung Ngurah Mayun Wirajaya, Ida Bagus Komang Mahardika, Yohanes Parlindungan Situmeang Agrotechnology Departement, Faculty of Agriculture, Warmadewa University, Jl. Terompong 24 Tanjung Bungkak, Denpasar-Bali, 80235, Indonesia Abstract The aim of this research is to determine the growth response and yield of several Black Soybean varieties in several variations of spacing. The experiment is a factorial experiment using a randomized block design consisting of two treatment factors, The first factor is the variety consisting of 3 levels: V1 = Detam 1, V2 = Detam 3 Prida, V3 = Detam 4 Prida. The second factor is spacing which consists of 3 levels: J1 = 20 x 20 cm, J2 = 20 x 30 cm, J3 = 20 x 40 cm. The interaction of varieties with plant spacing has a very significant effect on crop dry weight and seed oven per planting hole. Significant effect the yield seeds of dried and oven per hectare. The weight of oven dry seeds per planting hole and the highest yield of oven dried seeds per hectare was obtained in the interaction of Detam 1 varieties with spacing of 20 x 40 cm with was 33.79 g and 2.53 tons per hectare which increased 138.79% and 42.93% compared with the interaction of Detam 4 prida varieties with spacing of 20 x 20 cm with was 14.15 g and 1.77 tons per hectare. Keywords: black soybean, varieties, plant spacing Share Link | Plain Format | Corresponding Author (Made Sri Yuliartini)

84	Chemistry	ABS-679
Application of manure types in breeding several varieties of chili (Capsicum frutescens L.) Made Sri Yuliartini *, Anak Agung Ngurah Mayun Wirajaya, Axel Umbu Remu Zen Assegaf, Yohanes Parlindungan Situmeang Agrotechnologi Departement, Faculty of Agriculture, Warmadewa University, Jl. Terompong 24 Tanjung Bungkak, Denpasar-Bali, 80235, Indonesia * E-mail: yuliartinisri[at]yahoo.co.id Abstract The study aims to determine the effects of giving several types of manures on various chili pepper varieties. The experiment is a factorial experiment using a randomized block design consisting of two treatment factors, namely type of manure (K) of 3 levels: K1= Chicken Manure, K2= Cow Manure, K3= Rabbit Manure and type of varieties (V) of 4 levels: V1= Genie varieties, V2= Dewata varieties, V3= Sigantung varieties, V4= Gendut varieties. The interaction of varieties and type of manure as a very significant effect on the number of leaves and root fresh weight and significant effect on fresh weight and oven dry leaves. The highest fresh weight and oven dry weight was obtained in the interaction of Genie varieties with cow manure, namely 10.06 g and 1.68 g with increase 285.44% and 342.10% compared with the interaction of Sigantung varieties with cow manure, namely 2.61 g and 0.38 g. The highest fresh root weight was obtained in the interaction of Genie varieties with cow manure, which was 3.09 g or increased 286.25% compared to interaction between Dewata varieties and cow manure, which was 0.80 g. Genie varieties with cow manure types provide better chili seedling growth compared to Dewata varieties and Sigantung varieties. Keywords: Chili pepper breeding, varieties and manure Share Link | Plain Format | Corresponding Author (Made Sri Yuliartini)

85	Chemistry	ABS-935
Theoretical Investigation of Inclusion Complex between β-Cyclodextrin and Proton Pump Inhibitor Enantiomer Atthar Luqman Ivansyah1,2), Tety Sudiarti3), Soni Setiadji3), Citra Deliana Dewi Sundari4) 1) Master Program in Computational Science, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Jl. Ganesha No. 10, Bandung, West Java, 40132, Indonesia 2) Analytical Chemistry Research Group, Department of Chemistry, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Jl. Ganesha No. 10, Bandung, West Java, 40132, Indonesia 3) Department of Chemistry, UIN Sunan Gunung Djati Bandung, Jl. A.H. Nasution No.105, Bandung, West Java, 40164, Indonesia 4) Department of Chemistry Education, UIN Sunan Gunung Djati Bandung, Jl. Cimincrang, Cimenerang, Panyileukan, Bandung, West Java, 40292, Indonesia Abstract Theoretical investigation done in this research was used to explore the potency of β-Cyclodextrin for producing of high activity proton pump inhibitor. The host molecule used in this research is β-Cyclodextrin and the guest molecules are R/S-Omeprazole, R/S-Pantoprazole, R/S-Lansoprazole, dan R/S-Rabeboprazole. First, we did molecular docking study between host molecule and each of guest molecules by using ArgusLab version 4.0.1. Then, we optimized the structure from molecular docking result, by using Quantum Semiempiric Method implemented in Gaussian 09 Rev. D 01. Keywords: Molecular docking, Quantum Semiempiric, Gaussian 09 Rev. D01, Inclusion complex, β-Cyclodextrin, Proton Pump Inhibitor enantiomer Share Link | Plain Format | Corresponding Author (Atthar Luqman Ivansyah)

86	Chemistry	ABS-680
Chemical Analysis of Commercial Quail Laying Ration With Substitution of Fermentation Waste Bean Sprouts Ni Ketut Mardewi, Ni Ketut Sri Rukmini, I Gst. A. Dewi Seri Rejeki Animal Husbandry Department, Faculty of Agriculture, Warmadewa University. Jl. Terompong No.24, Tanjung Bungkak, Denpasar-Bali,80235,Indonesia Email: mardewiketut8[at]gmail.com Abstract Japanese quail (Coturnix-coturnix japonica) has great potential to be developed as an alternative source of cheap animal protein. Generally, quail breeders use commercial rations. The problem is that commercial rations are expensive. From an economic perspective, the cost of the ration is very high, reaching 70% of the total cost of production. To reduce the cost of ration and to maintain the nutritional quality, it is tried to substitute commercial rations using feed ingredients that have good nutritional content and the price is cheap by using waste bean sprouts. The purpose of this study was to determine the chemical quality of commercial quail laying rations substituted with fermented waste bean sprouts. The study used a completely randomized design (CRD). The treatment consisted of substituting 0%, 5%, 10% and 15% waste bean sprouts in the commercial quail laying ration. From the research results obtained that the substitution of fermented waste bean sprouts in commercial quail laying rations has a very significant effect (P<0.01) on dry matter, water content, ash content and organic matter. Substitution of waste bean sprouts in quail laying rations up to the level of 10% produces the lowest ash content and water content and meets the SNI standard, as well as the highest organic matter content of the ration. Keywords: Chemical Quality, Quail, Substitution, Waste Bean Sprouts Share Link | Plain Format | Corresponding Author (Ni Ketut Mardewi)

87	Chemistry	ABS-699
Utilization of fermented cassava skin ration on carcass weight and percentage of broiler carcass Melfiyanto Bili Mori, Ni Made Ayu Gemuh Rasa Astiti *, Ni Ketut Mardewi Faculty of Agriculture, Warmadewa University, Denpasar, Indonesia * E-mail: ayugemuh[at]gmail.com Abstract The purpose of this study was to determine the effect of fermented cassava peel flour on carcass weights and carcass parts of broilers. This study uses 45 broiler CP-707 strains. The design used was a completely randomized design with 3 replications. The treatments given were fermented cassava skin ration treatment consisting of 5 levels, namely: without fermented cassava skin flour (P0), fermented cassava skin flour 3% (P1), fermented cassava skin flour 6% (P2), fermented cassava skin flour 9 % (P3), and cassava peel flour fermented 12% (P4). The application of fermented cassava peel at 9% level in the 6-week broiler ration did not significantly influence all observed variables, but administration at the 6% level tended to increase carcass weight, chest weight, thigh weight, and wing weight. While the provision of fermented cassava peel flour at a rate of 12% in the ration had a significant effect in reducing carcass weight and weight of broilers at 6 weeks. The results showed that the fermentation of cassava peel flour in rations up to 6% tended to increase carcass weight, chest weight, thigh weight, and wing weight, whereas at 12% had a significant effect. in reducing carcass weight and wing weight of broilers at 6 weeks. Keywords: Broiler, fermentation, carcass, Cassava peel Share Link | Plain Format | Corresponding Author (NI MADE AYU GEMUH RASA ASTITI)

88	Chemistry	ABS-700
APPLICATION OF YEAST IN CONTROL OF GROWTH PATHOGENIC MOLD ON CHOCOLATE FRUIT Dalia Sukmawati1,2 , Z. Arman1, R. Hasanah1, M. Balqis1, A. Setiawan1, F. Tafrijiyyah1, R. Sari1, Tri Handayani Kurniati1, Atin Supiyani1, H. El Enshasy3,4 , Nining Betawati Prihantini5 1Biology Department, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia 2Universitas Negeri Jakarta Culture Collection, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia 3Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM), 81130 UTM, Skudai, Malaysia 4Departemen of Bioprocess and Polymer Engineering, School of Chemical and Energy Engineering, Faculty of Enginering, Universiti Teknologi Malaysia, 81310, Skudai, Johor, Malaysia 5Department of Biology, Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Indonesia, Kampus UI, Depok 16424, Indonesia Abstract Pathogenic mold can cause damage to chocolate plants such as rotting diseases in fruit. One of alternative that can be used to control pathogenic mold is using the yeast. Yeast has a cellulase enzyme that can degrade cellulose, where the mold cell walls are composed of semi-crystalline chitin, β-need and cellulose. This study aims to determine the yeast origin of fermented cocoa beans that produce cellulase enzymes and its potential as a control for pathogenic molds in chocolate fruit. Stages of research carried out were isolation of yeast from cocoa fermentation, screening of yeast isolates that produce cellulase enzymes, and testing of yeast antagonist against pathogenic molds on chocolate fruit. The results showed that there were 21 yeast isolates from the fermentation of cocoa beans, which five were able to produce cellulase enzymes namely yeast isolates C4.-3.3, C4.-3.13, C4.-4.9, C4.-4.10, and C4.-5.9. Yeast isolate C4.-4.10 is able to produce cellulase enzymes with an index of 0.32 U/mL. Five isolates that can produce cellulase enzymes were tested for antagonism against pathogenic molds in chocolate fruit. Keywords: Yeast, fermentation, phatogenic, chocolate Share Link | Plain Format | Corresponding Author (Dalia Sukmawati)

89	Chemistry	ABS-957
Bacteriocin Antimicrobial Isolation in Fish Soy Sauce Ni Made Darmadi, Dewa Gede Semara Edi, S.A.M.P. Suryani Study Program, Management Of Aquatic Resources, Faculty Of Agriculture Warmadewa University, Denpasar, Bali, Indonesia Abstract Fish sauce can be made from fermented fish . In fermentation plays a role Lactic Acid Bacteria (LAB). The role of lactic acid bacteria has a preservative effect because it can produce compounds that can inhibit the growth of various microbes. Besides lactic acid bacteria produce bacteriocin which also function as antimicrobials.The purpose of this study was to be able to isolate bacteriocin from fish sauce with SDS-PAGE electrophoresis. The study was conducted at the Laboratory of Agricultural Biotechnology Udayana University, Denpasar with a Descriptive Method. The research was carried out in sequence, namely, isolating lactic acid bacteria from fish sauce, Growing Lactic Acid Bacteria on Poor Nutrient Media so that its growth is depressed, because with life stressed Lactic Acid Bacteria can remove Bacteriocin as an Antimicrobial agent. With SDS-Page you will see separate proteins according to their weight, which are specific bands. The protein band which ranges from 17.0 kDa - 17.5 kDa is bacteriocin. Keywords: Bacteriocin, LAB, Fish Sauce Share Link | Plain Format | Corresponding Author (Ni Made Darmadi)

90	Chemistry	ABS-702
Quality Broiler Chicken Meat Fed with Rations Containing Golden Snail Flour as a Fish Meal Supplement Ni Ketut Etty Suwitari , Luh Suariani , Ni Made Yudiastari, Nyoman Kaca, Yan Tonga Department of Animal Husbandry, Faculty of Agriculture, Warmadewa University, Jl. Terompong 24, Denpasar, Bali. 80235. Indonesia. Email : suwitary62[at]yahoo.co.id Abstract Abstract. The purpose of this study was to determine the effect of replacing fish meal with golden snail flour on the quality of broiler chicken meat. This research was conducted using a Completely Randomized Design with 5 treatments (P0 = rations without golden snail flour (TKM), P1 = rations with 5% TKM), P2 = rations with 10% TKM, P3 = rations with 15% TKM, P4 = rations ration with 20% TKM) and three replications. Each replications consisted of 4 chickens. The variables observed were meat moisture content, cooking loss, water holding capacity, fat content, pH,total mikrobial (TPC) and ash content. The results showed that administration of golden snail flour in the ration had no significant effect on pH, moisture content, ash content, total microbial (TPC) and fat content of chicken meat, but significantly reduced cooking meat shrinkage and water holding capacity. Provision of Golden snail flour to a level of 10% can still be used as a substitute for fish meal in broiler rations. Keywords: Golden snail flour, Quality, Fish Meal, Broiler Share Link | Plain Format | Corresponding Author (Ni Ketut Etty Suwitari)

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